PMID: 2497763Feb 1, 1989Paper

Kinetics of infected cell appearance as a determinant of number of human immunodeficiency virus-1 infectious units

AIDS Research and Human Retroviruses
J M CoryF Rapp

Abstract

In order to optimize detection of human immunodeficiency virus-1 (HIV-1)-infected cells, the temporal appearance of virus antigens in newly infected H9 cell cultures was examined. Analyses were accomplished by indirect immunofluorescence labeling with each of 10 monoclonal antibodies and evaluation by flow cytometry. Of the antibodies examined, those specific for HIV-1 capsid protein p24, matrix protein p17, or their precursor molecule p55 allowed the earliest and most sensitive detection in infected cells fixed to allow detection of intracellular antigen. Discrimination of infected cells from uninfected cells was much less sensitive when three antibodies specific for HIV-1 glycoproteins were used to detect intracellular or cell surface antigen. In several experiments involving the time course of infection, we observed no differences in cell numbers between infected and uninfected H9 cultures initiated at identical cell concentrations. We hypothesized that it might be possible to quantitate infectious HIV-1 virions from the kinetics of infected cell appearance. Straight-line relationships between the log p24-positive cells and the time after infection were observed. These quantitative observations were employed to calculate the...Continue Reading

References

Mar 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P A LuciwJ A Levy
Mar 1, 1985·Cell·A B Rabson, M A Martin
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·T FolksK Sell
Jan 8, 1988·Science·B K Felber, G N Pavlakis
Jan 1, 1987·AIDS Research and Human Retroviruses·F Wong-StaalJ Ghrayeb

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Citations

Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D MathezJ Leibowitch
Feb 7, 2001·Clinical Microbiology Reviews·A Alvarez-BarrientosM Sánchez-Pérez
Jan 1, 1990·Cytometry·J M CoryB M Ohlsson-Wilhelm
Jul 1, 2011·Veterinary Immunology and Immunopathology·Melanie Ammersbach, Dorothee Bienzle
Sep 24, 2004·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Jutta K NeuenburgRobert M Grant
Oct 1, 1994·Clinical Microbiology Reviews·J J McSharry

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