Kinetics of lactose fermentation using a recombinant Saccharomyces cerevisiae strain

Biotechnology and Bioengineering
Martin JurascíkJ Markos

Abstract

This work presents a multi-route, non-structural kinetic model for interpretation of ethanol fermentation of lactose using a recombinant flocculent Saccharomyces cerevisiae strain expressing both the LAC4 (coding for beta-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces lactis. In this model, the values of different metabolic pathways are calculated applying a modified Monod equation rate in which the growth rate is proportional to the concentration of a key enzyme controlling the single metabolic pathway. In this study, three main metabolic routes for S. cerevisiae are considered: oxidation of lactose, reduction of lactose (producing ethanol), and oxidation of ethanol. The main bioprocess variables determined experimentally were lactose, ethanol, biomass, and dissolved oxygen concentrations. Parameters of the proposed kinetic model were established by fitting the experimental data obtained in a small lab-scale fermentor with the initial lactose concentrations ranging from 5 g/dm3 to 50 g/dm3. A very good agreement between experimental data and simulated profiles of the main variables (lactose, ethanol, biomass, and dissolved oxygen concentrations) was achieved.

References

Jul 3, 1999·Applied Microbiology and Biotechnology·L DominguesN Lima
Jul 23, 1999·Biotechnology and Bioengineering·L DominguesJ A Teixeira
Jul 19, 2001·Biotechnology and Bioengineering·L DominguesJ A Teixeira
Feb 6, 2004·Journal of Industrial Microbiology & Biotechnology·Luís G S LonghiArgimiro R Secchi

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Citations

Feb 18, 2011·Bioengineered Bugs·Lucília DominguesCarla Oliveira

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