Kinetics of metastatic breast cancer cell trafficking in bone

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Pushkar A PhadkeDanny R Welch

Abstract

In vivo studies have focused on the latter stages of the bone metastatic process (osteolysis), whereas little is known about earlier events, e.g., arrival, localization, and initial colonization. Defining these initial steps may potentially identify the critical points susceptible to therapeutic intervention. MDA-MB-435 human breast cancer cells engineered with green fluorescent protein were injected into the cardiac left ventricle of athymic mice. Femurs were analyzed by fluorescence microscopy, immunohistochemistry, real-time PCR, flow cytometry, and histomorphometry at times ranging from 1 hour to 6 weeks. Single cells were found in distal metaphyses at 1 hour postinjection and remained as single cells up to 72 hours. Diaphyseal arrest occurred rarely and few cells remained there after 24 hours. At 1 week, numerous foci (2-10 cells) were observed, mostly adjacent to osteoblast-like cells. By 2 weeks, fewer but larger foci (> or =50 cells) were seen. Most bones had a single large mass at 4 weeks (originating from a colony or coalescing foci) which extended into the diaphysis by 4 to 6 weeks. Little change (<20%) in osteoblast or osteoclast numbers was observed at 2 weeks, but at 4 to 6 weeks, osteoblasts were dramatically red...Continue Reading

References

Jun 3, 1977·Science·C R MundyJ G Bandelin
Dec 1, 1987·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A M ParfittR R Recker
Jan 1, 1987·British Journal of Cancer·R E Coleman, R D Rubens
Aug 1, 1982·The Journal of Clinical Endocrinology and Metabolism·A F StewartR Baron
Jan 1, 1994·Breast Cancer Research and Treatment·T YonedaG R Mundy
Mar 1, 1997·Clinical & Experimental Metastasis·V SungE W Thompson
Nov 15, 1997·Cancer·R E Coleman
Jul 20, 1999·Clinical & Experimental Metastasis·M LelekakisR L Anderson
Feb 3, 2000·Proceedings of the National Academy of Sciences of the United States of America·M YangR M Hoffman
Mar 24, 2000·Invasion & Metastasis·O H Sanchez-SweatmanG Singh
Apr 18, 2000·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J L HolleranL A Culp
Jun 14, 2000·Clinical & Experimental Metastasis·S F GoldbergD R Welch
Feb 24, 2001·Critical Reviews in Eukaryotic Gene Expression·J M Chirgwin, T A Guise
Feb 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Dengshun Miao, Andrew Scutt
Mar 14, 2002·The American Journal of Pathology·Antoinette WetterwaldMarco G Cecchini
Aug 3, 2002·Nature Reviews. Cancer·Gregory R Mundy
Dec 25, 2002·Clinical & Experimental Metastasis·Barbara O'Kane MurphyJ Graham Sharp
Jul 15, 2003·Clinical & Experimental Metastasis·John F Harms, Danny R Welch
Jan 27, 2004·Journal of Cellular Biochemistry·Andrea M MastroKristin Guttridge
Apr 16, 2004·The New England Journal of Medicine·G David Roodman
Jun 1, 2004·Clinical & Experimental Metastasis·John F HarmsDavid W Griggs
Jun 2, 2004·Nature Reviews. Cancer·Klaus Pantel, Ruud H Brakenhoff
Sep 29, 2004·Journal of Virology·Brian A Van TineLouise T Chow
Jan 28, 2005·Clinical & Experimental Metastasis·Robyn R MercerAndrea M Mastro
Feb 19, 2005·Current Treatment Options in Oncology·Allan Lipton
May 17, 2005·Cancer Cell·Conor C LynchMitsuru Futakuchi
Sep 6, 2005·Cancer Research·Christopher L HallEvan T Keller

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Citations

Dec 12, 2007·Cancer Metastasis Reviews·Karen M BussardAndrea M Mastro
Jun 11, 2008·Clinical & Experimental Metastasis·Ravi DhurjatiErwin A Vogler
Mar 19, 2009·Clinical & Experimental Metastasis·Linette Castillo-PichardoSuranganie Dharmawardhane
Jun 3, 2010·Clinical & Experimental Metastasis·Michelle M Martínez-MontemayorSuranganie Dharmawardhane
Dec 2, 2009·Journal of Mammary Gland Biology and Neoplasia·Karen M Bussard, Andrea M Mastro
Feb 22, 2007·Journal of the National Cancer Institute·Kevin T NashDanny R Welch
Mar 28, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Inna SerganovaRonald Blasberg
Jan 1, 2008·Journal of Oncology·Claudia CasarsaDanila Coradini
Sep 6, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mark A WatsonRebecca L Aft
Jul 20, 2007·Arthritis Research & Therapy·Mandeep S Virk, Jay R Lieberman
Dec 24, 2010·Breast Cancer Research : BCR·Yu-Chi ChenAndrea M Mastro
Mar 1, 2011·Chinese Journal of Cancer·Thomas M BodenstineDanny R Welch
Oct 10, 2006·Future Oncology·Benjamin D HedleyAnn F Chambers
Feb 9, 2012·International Journal of Breast Cancer·Donna M SosnoskiAndrea M Mastro
Apr 13, 2011·Journal of Cell Communication and Signaling·Osama Hussein, Svetlana V Komarova
Nov 3, 2007·Experimental Cell Research·Michelle KinderAndrea M Mastro
Jul 9, 2009·International Journal of Cancer. Journal International Du Cancer·Mick D EdmondsDanny R Welch
May 2, 2007·Journal of Cellular Biochemistry·Preeti KapoorHenry J Donahue
Sep 12, 2013·Journal of Cellular Physiology·Venkatesh KrishnanAndrea M Mastro
Jun 4, 2015·Journal of Cellular Biochemistry·Venkatesh KrishnanAndrea M Mastro
Aug 22, 2006·Biochemical and Biophysical Research Communications·Douglas R HurstRajeev S Samant
May 24, 2013·Adipocyte·Susan M MajkaDwight J Klemm
Mar 10, 2015·Clinical & Experimental Metastasis·Donna M SosnoskiAndrea M Mastro

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