Kinetics of minichromosome replication in Escherichia coli B/r.

Journal of Bacteriology
A C LeonardC E Helmstetter

Abstract

Replication control of the minichromosome pAL2 was found to differ from that of the chromosome in synchronously dividing populations of Escherichia coli B/r. Initiation of minichromosome replication took place at an increasing rate throughout synchronous growth. No coupling to initiation of chromosome replication was detected. Minichromosome replication was further examined in a dnaA5(Ts) temperature-sensitive initiation mutant. When cultures held at nonpermissive temperature (41 degrees C) for 60 min were shifted to permissive temperature (25 degrees C), initiation of both pAL2 and chromosome replication ensued in two waves spaced 25 to 35 min apart. Evidence is presented that minichromosomes terminate replication by passing slowly through a series of dimeric intermediate forms before reaching the closed circular monomeric form. The consequence of this slow passage as a rate-limiting step in the initiation reaction is discussed.

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Citations

Aug 1, 1986·Molecular & General Genetics : MGG·M YoshimotoG Tamura
Jun 26, 2009·Nucleic Acids Research·María Luisa Martínez-RoblesDora B Krimer
Feb 6, 2010·Molecular Microbiology·David F BruhnMichele M Klingbeil
Sep 1, 1982·Microbiological Reviews·N H Mendelson
Jan 1, 1987·Journal of Bacteriology·L J Koppes, K von Meyenburg
Aug 1, 1987·Journal of Bacteriology·C E Helmstetter, A C Leonard
Feb 1, 1985·Journal of Bacteriology·A C LeonardC E Helmstetter

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