Kinetics of mRNA delivery and protein translation in dendritic cells using lipid-coated PLGA nanoparticles

Journal of Nanobiotechnology
Hanzey YasarClaus-Michael Lehr

Abstract

Messenger RNA (mRNA) has gained remarkable attention as an alternative to DNA-based therapies in biomedical research. A variety of biodegradable nanoparticles (NPs) has been developed including lipid-based and polymer-based systems for mRNA delivery. However, both systems still lack in achieving an efficient transfection rate and a detailed understanding of the mRNA transgene expression kinetics. Therefore, quantitative analysis of the time-dependent translation behavior would provide a better understanding of mRNA's transient nature and further aid the enhancement of appropriate carriers with the perspective to generate future precision nanomedicines with quick response to treat various diseases. A lipid-polymer hybrid system complexed with mRNA was evaluated regarding its efficiency to transfect dendritic cells (DCs) by simultaneous live cell video imaging of both particle uptake and reporter gene expression. We prepared and optimized NPs consisting of poly (lactid-co-glycolid) (PLGA) coated with the cationic lipid 1, 2-di-O-octadecenyl-3-trimethylammonium propane abbreviated as LPNs. An earlier developed polymer-based delivery system (chitosan-PLGA NPs) served for comparison. Both NPs types were complexed with mRNA-mCherry a...Continue Reading

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Citations

Dec 19, 2019·Integrative Biology : Quantitative Biosciences From Nano to Macro·A ReiserJ O Rädler
Sep 13, 2020·International Journal of Molecular Sciences·Shuqin XuLu Zhang
Feb 18, 2021·Molecular Cancer·Yang WangXiawei Wei
Feb 24, 2020·Biotechnology Advances·Yuhua WengYuanyu Huang
Aug 10, 2021·Molecular Aspects of Medicine·Alysia CoxEun Ji Chung
Jul 14, 2020·ACS Applied Materials & Interfaces·Xiyu KeHai-Quan Mao

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Methods Mentioned

BETA
transfection
PBAE
dynamic light scattering
Scanning Electron Microscopy
electrophoresis
FCS
flow cytometry
scanning
transmission electron microscopy
transfect

Software Mentioned

FlowJo
GraphPad
Fiji
ImageJ
LAS X
Graph Pad Prism

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