Kinetics of multilayer immobilized enzyme-filter reactors: Behavior of urease-filter reactors in different buffers

Biotechnology and Bioengineering
L GoldsteinL Shemer

Abstract

A sequential model which describes the kinetic behavior of multilayer immobilized-enzyme filter reactors for systems obeying the Michaelis-Menten scheme and various types of inhibition by product and by substrate is proposed. The model was varified experimentaly using a single enzyme-filter-substrate system-urease bound to nylon-filter disks acting on urea-in different buffers to obtain the various inhibition patterns.

References

Jan 1, 1976·Methods in Enzymology·L Goldstein
Sep 1, 1979·Biotechnology and Bioengineering·L ShemerL Goldstein
Jul 1, 1977·Biotechnology and Bioengineering·B AtkinsonI Rousseau
Jul 1, 1977·Biotechnology and Bioengineering·B Atkinson, I Rousseau
Apr 2, 1975·Journal of the American Chemical Society·N D Jespersen
Jan 1, 1973·Advances in Enzyme Regulation·P H Jellinck, C R Lyttle
Mar 10, 1971·Biochimica Et Biophysica Acta·M Hanss, A Rey
Apr 1, 1953·Archives of Biochemistry and Biophysics·M C WALL, K J LAIDLER

Citations

Mar 25, 1992·Biotechnology and Bioengineering·A Schmidt-Steffen, E Staude
Aug 20, 1987·Biotechnology and Bioengineering·Y KobayashiN Yokoi
Dec 1, 1994·Applied Biochemistry and Biotechnology·Y Qin, J M Cabral

Related Concepts

Buffers
Nylons
Urease
Patterns
Filter - Medical Device

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