Kinetics of murine gammaherpesvirus 68 gene expression following infection of murine cells in culture and in mice

Journal of Virology
Rosemary RochfordR D Cardin

Abstract

A model system to study the pathogenesis of gammaherpesvirus infections is the infection of mice with murine gammaherpesvirus 68 (MHV-68). To define the kinetics of infection, we developed an RNase protection assay to quantitate gene expression from lytic (K3, Rta, M8, DNA polymerase [DNA pol], and gB) and candidate latency (M2, M3, M9, M11, ORF73, and ORF74) genes. All candidate latency genes were expressed during lytic infection of 3T3 cells. Four kinetic classes of transcripts were observed following infection of 3T3 cells: immediate-early (K3, Rta, M8, and ORF73), early (DNA pol), early-late (M3, M11, and ORF74), and late (M2, M9, and gB). To assess the kinetics of viral gene expression in vivo, lungs, spleens, and mediastinal lymph nodes (MLN) were harvested from MHV-68-infected mice. All transcripts were expressed between 3 and 6 days postinfection (dpi) in the lungs. In the spleen, K3, M3, M8, and M9 transcripts were expressed between 10 and 16 dpi when latency is established. The K3, M3, M8, M9, and M11 transcripts were detected in the MLN from 2 through 16 dpi. This is the first demonstration of MHV-68 gene expression in the MLN. Importantly, our data showed that MHV-68 has different kinetics of gene expression at diff...Continue Reading

References

Sep 1, 1992·The Journal of General Virology·N P Sunil-ChandraA A Nash
Dec 1, 1992·The Journal of General Virology·N P Sunil-ChandraA A Nash
Jun 1, 1994·Clinical Immunology and Immunopathology·R Rochford, D E Mosier
Oct 1, 1996·Trends in Microbiology·M K Offermann
Nov 1, 1996·The Journal of General Virology·E J UsherwoodA A Nash
Sep 1, 1996·The Journal of Experimental Medicine·R D CardinP C Doherty
Aug 1, 1997·Journal of Virology·H W VirginS H Speck
Jun 24, 1998·The Journal of Experimental Medicine·J P StewartT Nash
Aug 26, 1998·Trends in Microbiology·J P Simas, S Efstathiou
Feb 6, 1999·The Journal of General Virology·J P SimasS Efstathiou
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·S M HusainJ T Sample
Nov 26, 1999·The Journal of General Virology·G H WangJ I Cohen
Jan 22, 2000·Journal of Virology·S LiuS H Speck
Feb 9, 2000·The Journal of Experimental Medicine·C M ParryA Alcami
Jul 6, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·E FlañoM A Blackman
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·P G StevensonP J Lehner

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Citations

Jul 26, 2003·Folia Microbiologica·J Rajcáni, M Kúdelová
Aug 19, 2010·Immunologic Research·Ting-Ting WuRen Sun
Oct 4, 2002·Microbes and Infection·Philip G StevensonStacey Efstathiou
Jan 27, 2005·Viral Immunology·Nancy Gasper-Smith, Kenneth L Bost
May 22, 2002·Journal of Virology·Joo Wook AhnDagmar G Alber
Sep 20, 2002·Journal of Virology·Jason B WeinbergRosemary Rochford
Jun 14, 2003·Journal of Virology·Sofia MarquesJ Pedro Simas
Sep 13, 2003·Journal of Virology·DeeAnn Martinez-GuzmanRen Sun
Jun 16, 2005·Journal of Virology·Dirk P DittmerBlossom Damania
Nov 25, 2005·Journal of Virology·Dang Thi LuaTakashi Aoki
Dec 15, 2005·Journal of Virology·Ashley L SteedHerbert W Virgin
Nov 3, 2010·American Journal of Physiology. Lung Cellular and Molecular Physiology·Joshua S StoolmanBethany B Moore
Mar 17, 2005·PLoS Biology·Neil J BennettPhilip G Stevenson
Aug 22, 2007·PloS One·Nagendra N ThakurHeiko Adler
Mar 28, 2008·Scandinavian Journal of Immunology·R C ThomsonB M Dutia
Sep 5, 2008·Virology·Yn NguyenJason B Weinberg
Apr 3, 2002·The Journal of Clinical Investigation·Victor van BerkelHerbert W Virgin
Jun 17, 2005·Acta Microbiologica Et Immunologica Hungarica·J Rajćáni, M Kúdelová
Mar 20, 2004·Immunity·Jessica M BonamePhilip G Stevenson
Jun 15, 2010·Cell Host & Microbe·L Steven JohnsonHerbert W Virgin
Aug 2, 2005·Virology·Jason B WeinbergKatherine R Spindler
Oct 3, 2012·PLoS Pathogens·Bruno FredericoPhilip G Stevenson

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