PMID: 4905672Jan 1, 1970Paper

Kinetics of neutralization of bacteriophage f2 by rabbit gamma-G-antibodies

Proceedings of the National Academy of Sciences of the United States of America
M A DudleyD T Rowlands

Abstract

The neutralization of bacteriophage f2 by intact gammaG-immunoglobulin or fragments is first order with respect to both bacteriophage and antibody. Minimum values for the rate constants are of the order of 10(7)M(-1) sec(-1). The temperature dependence of the rates corresponds to the activation parameters: DeltaHdouble dagger = 6.7 kcal mole(-1) and DeltaSdouble dagger = -4 cal deg(-1) mole(-1) (gammaG-immunoglobulin); DeltaHdouble dagger = 8.0 kcal mole(-1) and DeltaSdouble dagger = -0.9 cal deg(-1) mole(-1) (5S pepsin fragment); and DeltaHdouble dagger = 13.3 kcal mole(-1) and DeltaSdouble dagger = 12 cal deg(-1) mole(-1) (3.5S fragment). The kinetic observations are consistent with the view that the binding of a single antibody molecule can bring about phage neutralization. There are two ways in which a single antibody molecule can affect neutralization: (1) binding at or near some critical site on the phage may block its function, (2) binding may disturb the general architectural design of the protein shell of the phage. Although the rate of neutralization varied directly with antibody size, consideration of the activation parameters speaks against the dependence of neutralization on simple steric factors. Addition of antib...Continue Reading

References

Dec 1, 1965·Immunochemistry·J W Goodman, J J Donch
Sep 1, 1966·Journal of Molecular Biology·K WeberW Konigsberg
Mar 10, 1956·Nature·F H CRICK, J D WATSON
Jun 20, 1959·Nature·J T FINCH, A KLUG
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Jan 1, 1960·Proceedings of the Society for Experimental Biology and Medicine·H B LEVY, H A SOBER
Aug 1, 1960·Archives of Biochemistry and Biophysics·A NISONOFFD L WOERNLEY

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Citations

Jun 1, 1972·Virology·S Lewenton-Kriss, B Mandel
Aug 6, 2000·Solid State Nuclear Magnetic Resonance·M A AramendíaF J Urbano
Dec 1, 1971·The Journal of Experimental Medicine·R W RosensteinJ W Uhr
Mar 1, 1972·Immunochemistry·C L Hornick, F Karuch

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