Kinetics of nucleotide-induced changes in the tryptophan fluorescence of the molecular chaperone Hsc70 and its subfragments suggest the ATP-induced conformational change follows initial ATP binding

Biochemistry
J H Ha, D B McKay

Abstract

The kinetics of nucleotide-induced changes of tryptophan fluorescence have been measured for recombinant bovine 70 kDa heat shock cognate protein (Hsc70), a 60 kDa subfragment (amino acid residues 1-554) which has ATPase and peptide binding activities, and a 44 kDa subfragment (residues 1-386) which has only ATPase activity. The fluorescence changes resulting from ATP binding to Hsc70 and the 60 kDa fragment are biphasic, and can be interpreted as arising from a two-step process in which ATP initially binds in a bimolecular reaction, followed by a conformational change of the protein-MgATP complex. Fluorescence changes resulting from ADP binding indicate a single-step, bimolecular process. Under single-cycle conditions of the ATPase reaction, a fluorescence change is observed whose rate constant correlates with product release in Hsc70, and with product release/ATP hydrolysis (which are kinetically indistinguishable under single-cycle conditions) in the 60 kDa fragment. These data support a scheme for Hsc70 in which a conformational transition is induced after initial ATP binding but prior to hydrolysis, and the reverse transition is induced by product release. The 60 kDa fragment shows behavior that is quantitatively similar t...Continue Reading

Citations

Jul 6, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L A Boyer, C L Peterson
Mar 24, 2005·Journal of Molecular Biology·Simone PoppJochen Reinstein
Jun 24, 2003·Journal of Molecular Biology·Marcus MayerJohannes Buchner
May 1, 1997·Nature Structural Biology·S RüdigerB Bukau
Sep 5, 2002·Molecular Microbiology·Sergey V Slepenkov, Stephan N Witt
Aug 15, 1997·The EMBO Journal·S TakayamaJ C Reed
Feb 13, 2001·Journal of Virology·C S SullivanJ M Pipas
Dec 21, 2005·FEBS Letters·Holger RaviolMatthias P Mayer
Dec 31, 2002·FEBS Letters·Fernando MoroArturo Muga
Jun 10, 2011·Chembiochem : a European Journal of Chemical Biology·Regina S SchweizerJochen Reinstein
Feb 16, 2010·Protein Expression and Purification·Wen-I LuoJames A Cowan
Feb 26, 1998·Cell·B Bukau, A L Horwich
Apr 28, 2016·Journal of Medicinal Chemistry·Matthew David CheesemanKeith Jones
Aug 9, 2016·Cell Chemical Biology·Zapporah T YoungJason E Gestwicki
Jul 13, 2016·Protein Expression and Purification·Pamela CappellettiLoredano Pollegioni
Aug 1, 1997·The Journal of Biological Chemistry·E UngewickellS E Holstein
Mar 14, 2000·The Journal of Biological Chemistry·J J Silberg, L E Vickery
Mar 28, 1997·The Journal of Biological Chemistry·N BenaroudjM M Ladjimi
Mar 4, 2020·Cells·Zarema AlbakovaAlexander M Sapozhnikov
Aug 17, 2002·The Journal of Biological Chemistry·Christoph J KluckMatthias P Mayer
Apr 30, 1999·Physiological Reviews·A L Fink
Jan 1, 2014·Frontiers in Molecular Biosciences·Rui Sousa
May 8, 2018·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Matthias P Mayer
May 30, 2003·The Journal of Biological Chemistry·Chia-Cheng ChouChwan-Deng Hsiao
Jan 19, 2010·Biochemistry·Stefka G TanevaArturo Muga
Jun 27, 1998·Journal of Molecular Biology·D KlostermeierJ Reinstein
Aug 10, 2010·Journal of Chemical Theory and Computation·Adrien NicolaïDaniel R Ripoll
Nov 20, 2020··Mohammad Kawsar Sharif SiamMohammad Umer Sharif Shohan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.