PMID: 3975504Jan 1, 1985

Kinetics of O2 uptake and release by red cells in stopped-flow apparatus: effects of unstirred layer

Respiration Physiology
R A HollandJ Piiper

Abstract

Using a stopped-flow apparatus, measurements were made of the velocity of uptake and release of oxygen by red cells of man, sheep and goat, three species of widely differing red cell size. The results were used to calculate resistances to O2 uptake provided by: (1) any unstirred layer (USL) outside the cells; (2) the cell membrane; and (3) the cell substance, in which the process of simultaneous diffusion and chemical reaction occurs. For O2 release, the USL was virtually abolished by using sufficient dithionite in the reactant buffer for it to diffuse up to the cell membrane and mop up O2 as it passes out of the cell. Hence, differences in the rate of O2 uptake and release allowed estimation of the resistance and thickness of the USL. Its thickness in the three species was between 0.7 and 0.9 micron; it provided at least 70% of the resistance to O2 uptake located outside the cell interior (cell membrane plus USL). Existence of the USL slows the uptake of O2 in the stopped-flow apparatus by a factor of at least 1.8 to 2.0.

References

May 1, 1977·Respiration Physiology·R A HollandJ Zubzanda
Jun 1, 1977·Biophysical Journal·M Gad-El-HakH Kutchal
Jul 1, 1975·New Zealand Veterinary Journal·G V Wallace
May 1, 1975·The Journal of General Physiology·S Fischkoff, J M Vanderkooi
Dec 1, 1968·Journal of the American Oil Chemists' Society·R BattinoW F Danforth
Oct 1, 1969·Respiration Physiology·J H Chamley, R A Holland
Mar 1, 1966·The Journal of General Physiology·R A Holland, R E Forster
May 1, 1967·The Journal of General Physiology·R I Sha'afiA K Solomon
May 1, 1964·The Journal of General Physiology·S L JOHNSON, J W WOODBURY
Jun 1, 1951·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·P NICOLSON, F J W ROUGHTON

Citations

Sep 1, 1988·Respiration Physiology·S C Hempleman, A T Gray
Apr 1, 1988·Respiration Physiology·C HookJ Piiper
Sep 1, 1991·Respiration Physiology·D C BetticherA Tempini
Dec 1, 1992·Respiration Physiology·B PelsterR B Reeves
Jan 1, 1989·Comparative Biochemistry and Physiology. A, Comparative Physiology·D C Betticher, J Geiser
Jan 27, 1999·Isotopes in Environmental and Health Studies·M A Wunder, Gerolf Gros
Jun 5, 2002·American Journal of Respiratory and Critical Care Medicine·Brian L GrahamDavid J Cotton
Jul 1, 1994·The Journal of Clinical Investigation·C C HsiaE R Weibel
Dec 1, 1990·Experientia·P Haab
Mar 29, 2012·Plant, Cell & Environment·Jakob SantnerFien Degryse
May 22, 2008·Expert Opinion on Investigational Drugs·J Gainer
May 22, 2004·Journal of Atherosclerosis and Thrombosis·Bradley T Heppner, Louis Wm Morgan
Dec 4, 2012·Respiratory Physiology & Neurobiology·A FoucquierB Sapoval
Dec 17, 2016·Cell and Tissue Research·E R Weibel
Feb 15, 2005·Pulmonary Pharmacology & Therapeutics·J GainerR J Murray
Jul 13, 2000·Critical Care Medicine·M SingerJ Gainer
Dec 1, 2006·Physiological Measurement·Martin A EdwardsPatrick R Unwin
Jan 23, 2015·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Georgios TsiavaliarisVolker Endeward
Jan 1, 1987·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·K YamaguchiJ Piiper
Mar 20, 2021·Artificial Cells, Nanomedicine, and Biotechnology·Meghna S JayaramanEvan C Unger

Related Concepts

Ampholytes
Diffusion
Diphosphoglyceric Acids
Red Cell Ghost
Erythrocytes
Goat
Eryhem
Dioxygen
Physiology
Dall Sheep

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