Kinetics of radioiodinated species in subcellular fractions from rat hearts following administration of iodine-123-labelled 15-(p-iodophenyl)-3-(R,S)-methylpentadecanoic acid (123I-BMIPP)

European Journal of Nuclear Medicine
S MorishitaT Nishimura

Abstract

It is recognized that iodine-123-labelled 15-(p-iodophenyl)-3-(R,S)-methylpentadecanoic acid (123I-BMIPP) slowly washes out of the myocardium. The mechanism for the washout was investigated in normal rat hearts by analyses of the subcellular distribution and lipid classes based on the BMIPP metabolism. Rat hearts were excised at 1-120 min after intravenous injection of 123I-BMIPP. After counting the radioactivity, the hearts were digested with Nagarse and homogenized, and then fractionated into the cytosolic, mitochondrial, microsomal and crude nuclear fractions by centrifugations. The radioactivity of each fraction was counted, and the lipid classes were analysed by radio-thin-layer chromatographic and high-performance liquid chromatographic methods. The heart uptake of 123I-BMIPP was maximal at 5 min (6.81%+/-0.36% ID/g), and 41% of the radioactivity disappeared within 120 min. The myocardial radioactivity was immediately distributed into the cytosolic, mitochondrial, microsomal and crude nuclear fractions. The distribution (%) of each fraction was almost identical from 5 min through 120 min. The cytosolic fraction was always the major site of radioactivity deposition (60%), and the time-activity curve of the cytosolic fracti...Continue Reading

References

Apr 11, 1992·International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology·J KroppH J Biersack
Jan 1, 1990·European Journal of Nuclear Medicine·F F KnappJ Kropp
Jan 1, 1989·European Journal of Nuclear Medicine·T R DeGradoS J Gatley
Jan 1, 1988·International Journal of Radiation Applications and Instrumentation. Part B, Nuclear Medicine and Biology·K R AmbroseF F Knapp
Jan 1, 1986·European Journal of Nuclear Medicine·F F KnappM M Goodman
Jan 1, 1985·International Journal of Nuclear Medicine and Biology·C A OttoH Lee
May 1, 1980·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J D Green, H T Narahara

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Citations

Jul 23, 1998·Annals of Nuclear Medicine·M OshimaS Furui
Jul 26, 2005·European Journal of Nuclear Medicine and Molecular Imaging·Hiroyuki KageyamaNagara Tamaki
Jul 7, 2011·Annals of Nuclear Medicine·Kenji FukushimaShuji Sakai
Apr 7, 2005·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Nagara Tamaki
May 20, 1998·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·A J Sinusas
Jul 18, 2006·International Journal of Cardiology·Yoshihiro J AkashiStefan D Anker
Jun 27, 2018·The Journal of Pharmacy and Pharmacology·Eri Minanimo-MutaTakeshi Kimura

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