Kinetics of single cells: observation and modeling of a stochastic process

Applied and Environmental Microbiology
C Pin, J Baranyi

Abstract

The successive generation times for single cells of Escherichia coli K-12 were measured as described by A. Elfwing, Y. LeMarc, J. Baranyi, and A. Ballagi (Appl. Environ. Microbiol. 70:675-678, 2004), and the histograms they generated were used as empirical distributions to simulate growth of the population as the result of the multiplication of its single cells. This way, a stochastic birth model in which the underlying distributions were measured experimentally was simulated. To validate the model, analogous bacterial growth curves were generated by the use of different inoculum levels. The agreement with the simulation was very good, proving that the growth of the population can be predicted accurately if the distribution of the first few division times for the single cells within that population is known. Two questions were investigated by the simulation. (i) To what extent can we say that the distribution of the detection time, i.e., the time by which a single-cell-generated subpopulation reaches a detectable level, can be identified with that of the lag time of the original single cell? (ii) For low inocula, how does the inoculum size affect the lag time of the population?

References

Feb 1, 1988·International Journal of Food Microbiology·B M Mackey, A L Kerridge
Nov 1, 1994·International Journal of Food Microbiology·J Baranyi, T A Roberts
Jun 1, 1996·Trends in Microbiology·A S Kaprelyants, D B Kell
Nov 14, 1997·Journal of Applied Microbiology·P J StephensT J Humphrey
Sep 15, 1998·Journal of Theoretical Biology·J Baranyi
Apr 1, 2000·Applied and Environmental Microbiology·J C AugustinV Carlier
Jun 9, 2001·Journal of Theoretical Biology·J Baranyi, C Pin
Jan 5, 2002·International Journal of Food Microbiology·T P RobinsonB M Mackey
Apr 6, 2002·International Journal of Food Microbiology·József Baranyi
Jun 14, 2003·Letters in Applied Microbiology·K FrancoisJ Debevere
Nov 11, 2003·Journal of Microbiological Methods·Aline MétrisJózsef Baranyi
Feb 10, 2004·Applied and Environmental Microbiology·A ElfwingA Ballagi
Nov 9, 2004·Journal of Theoretical Biology·Zoltán KutalikJózsef Baranyi
Feb 3, 2005·PLoS Biology·Eric J StewartFrançois Taddei
Apr 28, 2005·International Journal of Food Microbiology·A MétrisJ Baranyi
Apr 28, 2005·International Journal of Food Microbiology·A R StandaertJ F Van Impe
Jun 4, 2005·Applied and Environmental Microbiology·L GuillierJ-C Augustin
Aug 11, 2005·International Journal of Food Microbiology·Zoltán KutalikJózsef Baranyi

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Citations

Oct 23, 2008·Lab on a Chip·S Elizabeth HulmeGeorge M Whitesides
Oct 1, 2011·Critical Reviews in Food Science and Nutrition·Micha Peleg, Maria G Corradini
Jun 14, 2013·Journal of the Royal Society, Interface·Evgeny B StukalinSean X Sun
Oct 6, 2009·Applied and Environmental Microbiology·J S AguirreG D García de Fernando
Aug 4, 2009·Applied and Environmental Microbiology·P K Malakar, G C Barker
Sep 5, 2006·Applied and Environmental Microbiology·A MétrisJ Baranyi
Nov 17, 2009·Applied and Environmental Microbiology·Joseph HorowitzMicha Peleg
Feb 26, 2008·Applied and Environmental Microbiology·Carmen Pin, József Baranyi
Jan 29, 2013·Applied and Environmental Microbiology·Konstantinos P Koutsoumanis, Alexandra Lianou
Apr 7, 2006·Applied and Environmental Microbiology·M D'ArrigoC Pin
Jan 1, 2014·BMC Systems Biology·Daniel MadarUri Alon
Apr 18, 2013·International Journal of Food Microbiology·Juan S AguirreGonzalo D García de Fernando
Feb 24, 2016·International Journal of Food Microbiology·Juan S Aguirre, Konstantinos P Koutsoumanis
Jun 5, 2012·Mathematical Biosciences·J Pérez-VelázquezS E Lindow
Sep 28, 2007·Journal of Applied Microbiology·P N SkandamisG-J E Nychas
Sep 14, 2007·Journal of Applied Microbiology·P M BeckerP G van Wikselaar
Oct 23, 2010·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Jun TeramotoAkira Ishihama
Apr 14, 2016·Current Biology : CB·Bram CerulusKevin J Verstrepen
Nov 3, 2010·International Journal of Radiation Biology·Wayne S Kendal
Jul 15, 2016·International Journal of Food Microbiology·Konstantinos P Koutsoumanis, Zafiro Aspridou
Dec 17, 2014·Food Microbiology·Zafiro Aspridou, Konstantinos P Koutsoumanis
Sep 15, 2016·Critical Reviews in Food Science and Nutrition·Tian DingDong-Hong Liu
Apr 6, 2017·BMC Systems Biology·Athanasios D BalomenosElias S Manolakos
Apr 8, 2014·Applied and Environmental Microbiology·Christina L LewisAndre G Senecal
Jun 15, 2014·Applied and Environmental Microbiology·Antonio A AlonsoConstantinos Theodoropoulos
Apr 25, 2019·Journal of the Royal Society, Interface·A BarizienCharles N Baroud
Mar 19, 2021·Scientific Reports·Chiara Enrico BenaCarla Bosia
Nov 23, 2021·Frontiers in Microbiology·Simen Akkermans, Jan F M Van Impe

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