Kinetics of Sugar Transport and Phosphorylation Influence Glucose and Fructose Cometabolism by Zymomonas mobilis.

Applied and Environmental Microbiology
C ParkerT Conway

Abstract

The competitive inhibition of fructokinase by glucose has been proposed as the mechanism by which Zymomonas mobilis preferentially consumes glucose from mixtures of glucose and fructose and accumulates fructose when growing on sucrose. In this study, incorporation of radioactive fructose into biomass was used as a measure of fructose catabolism. It was determined that the rate of fructose incorporation by Z. mobilis CP4 was somewhat lower in the presence of an equimolar concentration of glucose but that the inhibition of fructokinase by glucose was not nearly as severe in vivo as was predicted from in vitro studies. Interestingly, addition of glucose to a culture of Z. mobilis CP4-M2, a glucokinaseless mutant, resulted in an immediate and nearly complete inhibition of fructose incorporation. Furthermore, addition of nonmetabolizeable glucose analogs had a similar effect on fructose catabolism by the wild-type Z. mobilis CP4, and fructose uptake by Z. mobilis CP4-M2 was shown to be severely inhibited by equimolar amounts of glucose. These results suggest that competition for fructose transport plays an important role in preferential catabolism of glucose from sugar mixtures. Indeed, the apparent K(infm) values for sugar uptake b...Continue Reading

References

Mar 1, 1977·Bacteriological Reviews·J Swings, J De Ley
Sep 1, 1992·FEMS Microbiology Reviews·T Conway
Jan 1, 1985·Applied and Environmental Microbiology·A A Dimarco, A H Romano
Feb 1, 1987·Applied and Environmental Microbiology·T ConwayL O Ingram

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Citations

Dec 17, 2009·Applied Biochemistry and Biotechnology·Barry BochnerSteven D Brown
Jul 27, 1999·Journal of Bacteriology·E DoukaC Drainas
Mar 1, 2012·BMB Reports·Parisutham VinuselviCheol-Min Ghim
Jan 15, 2015·Applied Microbiology and Biotechnology·Kun ZhangHong Feng
Jan 27, 2015·Applied Biochemistry and Biotechnology·Alba RomeroSergio Sánchez
Jun 21, 2013·Organic & Biomolecular Chemistry·D Tyler McQuadePeter H Seeberger
Mar 30, 2006·Biotechnology and Bioengineering·Mehmet M AltintasDhinakar S Kompala
May 22, 2010·BMC Evolutionary Biology·Amy L Wilson-O'BrienSuzanne Rogers

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