Kinetics of the invasion and egress processes of Babesia divergens, observed by time-lapse video microscopy

Scientific Reports
Elena SevillaEstrella Montero

Abstract

Based on confocal fluorescence and bright field video microscopy, we present detailed observations on the processes of invasion and egress of erythrocytes by the apicomplexan parasite Babesia divergens. Time-lapse images reveal numerous unexpected findings associated with the dynamics of B. divergens and its ability to manipulate the erythrocyte during both processes in its asexual cycle under in vitro conditions. Despite the speed at which these processes occur and the small size of the parasite, we capture infective merozoites moving vigorously and causing striking deformations in the erythrocyte's plasma membrane during an active invasion. We also observed intraerythrocytic dynamic stages as paired pyriforms, double paired pyriforms, tetrads, unattached pyriform sister cells and multiple parasite stages resulting in the release of large numbers of merozoites over a short period. Of considerable interest is that time-lapse images reveal a novel mechanism of egress used by B. divergens to exit the human erythrocyte. The release occurs when B. divergens parasites establish contacts with the plasma membrane of the erythrocyte from within, before exiting the cell. Visualization and analysis of the images enabled us to obtain usef...Continue Reading

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Citations

Jul 5, 2019·Pathogens·Cheryl A LoboMarilis Rodriguez
Aug 20, 2019·PLoS Neglected Tropical Diseases·Luis Miguel GonzálezEstrella Montero
Jun 15, 2019·Life Science Alliance·Jose ThekkiniathChoukri Ben Mamoun
Feb 6, 2020·MSystems·Hao JiangYang Zhang
Feb 13, 2021·Parasite : Journal De La Société Française De Parasitologie·Xinlei YanXun Suo
Jan 13, 2021·Pathogens·Monica Florin-ChristensenLeonhard Schnittger
Aug 21, 2021·Frontiers in Cellular and Infection Microbiology·Vitomir DjokicNikhat Parveen

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Methods Mentioned

BETA
transmission electron microscopy
light microscopy

Software Mentioned

GraphPad Prims
Leica AF
ImageJ
SPSS

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Babesiosis (ASM)

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.

Babesiosis

Babesiosis is caused by parasites of the genus babesia, which are transmitted in nature by the bite of an infected tick. Discover the latest research on babesiosis here.