Kinetics of the oxidation of reduced Cu,Zn-superoxide dismutase by peroxymonocarbonate.

Free Radical Biology & Medicine
Kalina RanguelovaRonald P Mason

Abstract

Kinetic evidence is reported for the role of the peroxymonocarbonate, HOOCO(2)(-), as an oxidant for reduced Cu,Zn-superoxide dismutase-Cu(I) (SOD1) during the peroxidase activity of the enzyme. The formation of this reactive oxygen species results from the equilibrium between hydrogen peroxide and bicarbonate. Recently, peroxymonocarbonate has been proposed to be a key substrate for reduced SOD1 and has been shown to oxidize SOD1-Cu(I) to SOD1-Cu(II) much faster than H(2)O(2). We have reinvestigated the kinetics of the reaction between SOD1-Cu(I) and HOOCO(2)(-) by using conventional stopped-flow spectrophotometry and obtained a second-order rate constant of k=1600±100M(-1)s(-1) for SOD1-Cu(I) oxidation by HOOCO(2)(-). Our results demonstrate that peroxymonocarbonate oxidizes SOD1-Cu(I) to SOD1-Cu(II) and is in turn reduced to the carbonate anion radical. It is proposed that the dissociation of His61 from the active site Cu(I) in SOD-Cu(I) contributes to this chemistry by facilitating the binding of larger anions, such as peroxymonocarbonate.

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Citations

Oct 23, 2013·Canadian Journal of Physiology and Pharmacology·Naif AljuhaniArno G Siraki
Sep 4, 2015·Free Radical Research·Madia TrujilloRafael Radi
Apr 2, 2014·Chemical Reviews·Yuewei ShengJoan Selverstone Valentine
Nov 10, 2012·Free Radical Biology & Medicine·Ronald P MasonKalina Ranguelova
Nov 10, 2012·Free Radical Biology & Medicine·Stefan I Liochev, Irwin Fridovich
Oct 4, 2018·Acta Crystallographica. Section F, Structural Biology Communications·Jahaun AzadmaneshGloria E O Borgstahl
Dec 19, 2015·Physiological Reviews·Xin Gen LeiElias S J Arnér
Aug 28, 2018·Chemical Reviews·Saba ParvezYimon Aye

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