PMID: 9182760May 30, 1997Paper

Kinetochore localization of murine Bub1 is required for normal mitotic timing and checkpoint response to spindle damage

Cell
Stephen S Taylor, Frank McKeon

Abstract

The mitotic checkpoint ensures proper chromosome segregation by delaying anaphase until chromosomes are aligned on the spindle. Following prolonged spindle damage, however, cells eventually exit mitosis and undergo apoptosis. We show here that a murine homolog of the yeast mitotic checkpoint gene BUB1 localizes to the kinetochore during mitosis. By expressing a dominant-negative mutant, we show that mBub1 is not only required for the checkpoint response to spindle damage, but acts in the timing of a normal mitosis. In addition, when mBub1 function is compromised, cells escape apoptosis and continue cell cycle progression, despite leaving mitosis with a disrupted spindle. These data demonstrate a role for kinetochore-associated mBub1 in regulating exit from mitosis, and suggest functional links between the mitotic checkpoint and subsequent apoptotic events in G1.

References

Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Gossen, H Bujard
Aug 9, 1991·Cell·R Li, A W Murray
Jan 1, 1991·Cold Spring Harbor Symposia on Quantitative Biology·J R McIntosh
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A L KungR T Schimke
Nov 3, 1989·Science·L H Hartwell, T A Weinert
May 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·F M DavisP N Rao
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·Y MoroiE M Tan
Nov 1, 1995·The Journal of Cell Biology·K G Hardwick, A W Murray
Sep 1, 1993·The Journal of Cell Biology·G J Gorbsky, W A Ricketts
Feb 1, 1995·Current Opinion in Genetics & Development·A W Murray
Feb 16, 1995·Nature·X Li, R B Nicklas
Dec 1, 1994·Current Opinion in Cell Biology·A Murray
Nov 1, 1994·The Journal of Cell Biology·P R Andreassen, R L Margolis
Dec 1, 1994·Molecular and Cellular Biology·B T RobertsM A Hoyt
Dec 16, 1994·Science·L H Hartwell, M B Kastan
Sep 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·S M KerrH J Cooke
Apr 1, 1996·The Journal of Cell Biology·W A Wells, A W Murray
Oct 11, 1996·Science·Y Li, R Benezra
Aug 1, 1996·Molecular Biology of the Cell·F Pangilinan, F Spencer
Dec 1, 1996·Current Opinion in Cell Biology·A D Rudner, A W Murray
Jan 31, 1997·Science·R B Nicklas
Apr 1, 1995·Trends in Cell Biology·G J Gorbsky

❮ Previous
Next ❯

Citations

Mar 21, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·D J Clarke, J F Giménez-Abián
Jul 13, 2000·Cell Motility and the Cytoskeleton·M S CampbellG J Gorbsky
Sep 19, 2000·Genes, Chromosomes & Cancer·A GemmaS Kudoh
Oct 17, 2002·The Anatomical Record·Michael F DolanRobin Kolnicki
Jul 12, 2002·Cell Motility and the Cytoskeleton·Julie C CanmanGuowei Fang
Jan 25, 2002·Journal of Cellular Physiology·Katya RavidMatthew R Jones
Feb 18, 2010·Chromosoma·Frederick G WesthorpeStephen S Taylor
Mar 21, 2013·Chromosoma·Hironori Funabiki, David J Wynne
Jun 27, 2012·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Tetsuya Hori, Tatsuo Fukagawa
Dec 12, 2007·Investigational New Drugs·Ashish AggarwalSharon Lobert
Apr 22, 2010·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Ting ZhouShixuan Wang
May 24, 2005·Experimental Cell Research·Loleta HarrisRakesh Goorha
Jul 24, 2003·Journal of Theoretical Biology·Natalia L KomarovaMartin A Nowak
Feb 4, 1998·Trends in Genetics : TIG·K G Hardwick
Oct 22, 2003·Archives of Medical Research·Carla S Lopes, Claudio E Sunkel
Dec 4, 2001·Biology of the Cell·J L MallerB J Tunquist
May 4, 2002·Molecular and Cellular Endocrinology·James L MallerBrian J Tunquist
Mar 18, 2000·Toxicology Letters·P A JohnsonK W Caldecott
Jan 13, 1998·Current Opinion in Cell Biology·O Cohen-Fix, D Koshland
Jan 13, 1998·Current Opinion in Cell Biology·P K SorgerA A Hyman
Dec 22, 1999·Current Opinion in Cell Biology·W Zachariae
Jan 16, 1999·Current Opinion in Biotechnology·S A Courtneidge, G D Plowman
Feb 19, 2000·Current Opinion in Genetics & Development·D J Burke
May 14, 1999·Current Opinion in Structural Biology·R Heald, C E Walczak
Sep 22, 2001·Current Biology : CB·B M Stern, A W Murray
Sep 19, 1998·Current Biology : CB·D A Wolf, P K Jackson
Mar 31, 2000·Trends in Cell Biology·R D Gardner, D J Burke
Aug 15, 1998·Trends in Cell Biology·C L Rieder, E D Salmon
Apr 27, 1999·Trends in Biochemical Sciences·K Nasmyth
Aug 19, 2000·Molecular Cell·G PereiraE Schiebel
Feb 28, 2003·Ageing Research Reviews·Thomas V GetchellMarilyn L Getchell
Apr 12, 2008·Cell Death and Differentiation·H VakifahmetogluB Zhivotovsky

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.