Kinetochore-microtubule error correction is driven by differentially regulated interaction modes

Nature Cell Biology
Maria KalantzakiTomoyuki U Tanaka

Abstract

For proper chromosome segregation, sister kinetochores must interact with microtubules from opposite spindle poles (bi-orientation). To establish bi-orientation, aberrant kinetochore-microtubule attachments are disrupted (error correction) by aurora B kinase (Ipl1 in budding yeast). Paradoxically, during this disruption, new attachments are still formed efficiently to enable fresh attempts at bi-orientation. How this is possible remains an enigma. Here we show that kinetochore attachment to the microtubule lattice (lateral attachment) is impervious to aurora B regulation, but attachment to the microtubule plus end (end-on attachment) is disrupted by this kinase. Thus, a new lateral attachment is formed without interference, then converted to end-on attachment and released if incorrect. This process continues until bi-orientation is established and stabilized by tension across sister kinetochores. We reveal how aurora B specifically promotes disruption of the end-on attachment through phospho-regulation of kinetochore components Dam1 and Ndc80. Our results reveal fundamental mechanisms for promoting error correction for bi-orientation.

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Nov 4, 2015·Frontiers in Oncology·Veronica Krenn, Andrea Musacchio
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Apr 15, 2021·The Journal of Cell Biology·Harinath DoodhiTomoyuki U Tanaka

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Methods Mentioned

BETA
two-hybrid
PCR
FACS
fluorescence microscopy

Software Mentioned

Aurora
Chroma
SoftWoRx
Volocity
Improvision
Prism ( Graph Pad )

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