Kinetochore proteins suppress neuronal microtubule dynamics and promote dendrite regeneration.

Molecular Biology of the Cell
J Ian HertzlerMelissa M Rolls

Abstract

Kinetochores connect centromeric chromatin to spindle microtubules during mitosis. Neurons are postmitotic, so it was surprising to identify transcripts of structural kinetochore (KT) proteins and regulatory chromosome passenger complex (CPC) and spindle assembly checkpoint (SAC) proteins in Drosophila neurons after dendrite injury. To test whether these proteins function during dendrite regeneration, postmitotic RNA interference (RNAi) was performed and dendrites or axons were removed using laser microsurgery. Reduction of KT, CPC, and SAC proteins decreased dendrite regeneration without affecting axon regeneration. To understand whether neuronal functions of these proteins rely on microtubules, we analyzed microtubule behavior in uninjured neurons. The number of growing plus, but not minus, ends increased in dendrites with reduced KT, CPC, and SAC proteins, while axonal microtubules were unaffected. Increased dendritic microtubule dynamics was independent of dual leucine zipper kinase (DLK)-mediated stress but was rescued by concurrent reduction of γ-tubulin, the core microtubule nucleation protein. Reduction of γ-tubulin also rescued dendrite regeneration in backgrounds containing kinetochore RNAi transgenes. We conclude tha...Continue Reading

References

Jan 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·S HenikoffB van Steensel
Dec 14, 2001·Proceedings of the National Academy of Sciences of the United States of America·X MorinW Chia
Nov 14, 2002·Molecular Biology of the Cell·Ingrid Brust-Mascher, Jonathan M Scholey
May 2, 2007·Neural Development·Melissa M RollsChris Q Doe
May 25, 2007·FEBS Letters·Claudio CiferriArsen Petrovic
Jan 10, 2008·Current Opinion in Cell Biology·Tomoyuki U Tanaka, Arshad Desai
Mar 7, 2008·Nature Reviews. Molecular Cell Biology·Anna Akhmanova, Michel O Steinmetz
Aug 1, 2008·Molecular Biology of the Cell·Michelle C StoneMelissa M Rolls
Jan 24, 2009·Science·Marc HammarlundMichael Bastiani
Aug 13, 2010·Current Opinion in Cell Biology·Karen E Gascoigne, Iain M Cheeseman
Oct 6, 2010·The Journal of Cell Biology·Xin XiongCatherine A Collins
Feb 12, 2011·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·John Tooley, P Todd Stukenberg
Jan 13, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Xin Xiong, Catherine A Collins
Jun 16, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Patricia R GoodwinPeter Juo
Jun 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Li ChenMelissa M Rolls
Nov 24, 2012·Current Biology : CB·Pablo Lara-GonzalezStephen S Taylor
Nov 24, 2012·Nature Reviews. Molecular Cell Biology·Mar CarmenaWilliam C Earnshaw
Feb 12, 2013·Current Biology : CB·Kari BarlanVladimir I Gelfand
Feb 19, 2013·Journal of Cell Science·Dileep Varma, E D Salmon
Mar 27, 2013·The Journal of Cell Biology·Karen E Gascoigne, Iain M Cheeseman
Jan 15, 2014·Cell Reports·Michelle C StoneMelissa M Rolls
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
May 9, 2014·Molecular Biology of the Cell·Michelle M NguyenMelissa M Rolls
Jul 6, 2014·Cold Spring Harbor Perspectives in Biology·Iain M Cheeseman
Sep 16, 2014·Nature Communications·Tatjana KleeleThomas Misgeld
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Mar 10, 2015·Nature Methods·Daehwan KimSteven L Salzberg
Oct 21, 2015·Current Biology : CB·Andrea Musacchio
Nov 4, 2015·Frontiers in Oncology·Veronica Krenn, Andrea Musacchio
Nov 10, 2015·Protein Science : a Publication of the Protein Society·Dan YuXiaokun Shu
Nov 13, 2015·Nature Reviews. Molecular Cell Biology·Anna Akhmanova, Michel O Steinmetz
Jan 29, 2016·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Kah Wai YauCasper C Hoogenraad
Feb 16, 2016·Trends in Cell Biology·Ines A DrinnenbergHarmit S Malik
Feb 19, 2016·Cytoskeleton·Peter W BaasLanfranco Leo
Mar 8, 2016·Current Opinion in Cell Biology·Banafsheh Etemad, Geert J P L Kops

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Citations

Mar 12, 2021·Neuroscience Letters·Alexis T WeinerMelissa M Rolls

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Methods Mentioned

BETA
RNASeq
laser capture microdissection
fluorescence microscopy
ubiquitination
transgenic

Software Mentioned

HISAT2
FASTQC
Samtools
Zen
DESeq2
dmel
GraphPad Prism
FIJI

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