PMID: 6162099Dec 1, 1980Paper

Kinetoplast DNA and RNA of Trypanosoma brucei

Molecular and Biochemical Parasitology
A M Simpson, L Simpson

Abstract

Kinetoplast DNA (kDNA) and kinetoplast RNA (kRNA) were isolated from bloodstream and procyclic culture forms of two clonal strains of Trypanosoma brucei. No differences were observed in kDNA (maxicircle) restriction profiles between bloodstream or procyclic culture forms of the same strain. Some differences were observed in kDNA maxicircle restriction sites between the two strains. Buoyant density analysis of Pst I digested kDNA showed the release of a minor low density band representing unit length linearized maxicircle DNA. Pst I or Bam H1-linearized maxicircle DNA was isolated by the Hoechst 33258 dye--CsCl method and a restriction enzyme map of the maxicircle was constructed. Closed monomeric minicircles released from kDNA networks by sonication sedimented with a molecular size of around 1100 base pairs. A substantial minor length heterogeneity was evident in acrylamide gel electrophoresis of once cut minicircles. Several minicircle sequence classes and two Hind III maxicircle fragments representing approx. 50% of the maxicircle were cloned in the bacterial plasmid, pBR322, in Escherichia coli. A purified kinetoplast-mitochondrion fraction was isolated from procyclic culture forms by the Renografin flotation method. The maj...Continue Reading

References

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Citations

Oct 1, 1982·Molecular and Biochemical Parasitology·A M SimpsonL Livingston
Oct 1, 1983·Molecular and Biochemical Parasitology·K Stuart
Jul 1, 1984·Molecular and Biochemical Parasitology·J M NtambiP T Englund
Oct 1, 1984·Molecular and Biochemical Parasitology·B J JohnsonJ E Donelson
Jun 1, 1984·Molecular and Biochemical Parasitology·T W Spithill, R J Grumont
Mar 1, 1986·Molecular and Biochemical Parasitology·D P Jasmer, K Stuart
Mar 1, 1986·Molecular and Biochemical Parasitology·D P Jasmer, K Stuart
Jul 1, 1986·Molecular and Biochemical Parasitology·U G Lopes, D F Wirth
Jan 15, 1988·Molecular and Biochemical Parasitology·J SternbergL Jenni
May 15, 1989·Molecular and Biochemical Parasitology·J SternbergA Tait
Jul 16, 2002·International Journal for Parasitology·Achim SchnauferKen Stuart
Apr 16, 2003·FEMS Microbiology Letters·Maria Cristina M MottaMarc Thiry
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M L MuhichA M Simpson
Oct 1, 1988·Experimental Parasitology·J D Berman, M Grogl
Aug 1, 1987·Molecular and Cellular Biology·M R Mowatt, C E Clayton

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