Kininogen binding to the surfaces of macrophages

International Immunopharmacology
Anna BarbaszAndrzej Kozik

Abstract

Kinin generation may be initiated on the cell surfaces via a primary kininogen docking which has been characterized for endothelial cells, platelets, neutrophils, astrocytes and smooth muscle cells. In this work we describe the adsorption of biotin-labeled human kininogens by murine RAW 264.7 macrophages and human U-937 monocytes/macrophages. Both cell types strongly bound high molecular mass kininogen (HK) in a zinc-ion dependent manner with the dissociation constants of 9.1 nM and 3.3 nM, respectively, and the binding capacities of 46 fmol and 71 fmol per million of respective cells. The HK binding was quenched by 50% by antibodies against Mac-1, gC1qR and uPAR proteins indicating that these macrophage surface receptors are involved in the HK adsorption. A significant increase of HK binding was observed after cell activation with phorbol myristate acetate. Our results suggest that macrophages, similarly to neutrophils, may supply kininogens to the inflammatory foci to support the local kinin production at these sites.

References

Jun 1, 1976·The Journal of Experimental Medicine·P RalphK Nilsson
Jul 1, 1989·The Journal of Clinical Investigation·E J GustafsonR W Colman
Oct 15, 1987·Thrombosis Research·D A JohnsonA J Barrett
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·A A HasanA H Schmaier
Sep 10, 1999·Clinical Immunology : the Official Journal of the Clinical Immunology Society·K JosephA P Kaplan
Jun 13, 2000·The Journal of Biological Chemistry·T RennéW Müller-Esterl
Jul 21, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M R GyetkoT J Standiford
Apr 18, 2001·International Archives of Allergy and Immunology·A P KaplanB Ghebrehiwet
Feb 14, 2002·The Journal of Allergy and Clinical Immunology·Allen P KaplanMichael Silverberg
Aug 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Jing-Chuan ZhangKeith R McCrae
Sep 4, 2003·The Journal of Biological Chemistry·Triantafyllos ChavakisKlaus T Preissner
Nov 5, 2003·Thrombosis and Haemostasis·Lawrence P FernandoAllen P Kaplan
Dec 24, 2003·Thrombosis and Haemostasis·Kusumam JosephAllen P Kaplan
Feb 12, 2005·Advances in Immunology·Kusumam Joseph, Allen P Kaplan
Jun 18, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Anthony N FernandoAllen P Kaplan
Aug 12, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Mohammad M KhanRobert W Colman

❮ Previous
Next ❯

Citations

Oct 10, 2008·Journal of Proteome Research·Elena AlbèDimitri T Azar
Oct 10, 2013·The Journal of Biological Chemistry·Ya-Chun ChenKenji Okuse
Jun 12, 2010·Biological Chemistry·Julius F Varano della VergilianaGeoffrey A Stewart
May 5, 2012·Neurochemistry International·Ibeth Guevara-Lora
Jul 26, 2011·Biochimie·Andrezza J GozzoMariana S Araújo
May 16, 2013·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Elena KashubaLynn Cawkwell
Mar 30, 2010·Biochimie·Gilles LalmanachHans Fritz
Apr 2, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aizhen YangYi Wu
Jul 27, 2017·Frontiers in Physiology·Guacyara Motta, Ivarne L S Tersariol

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.