Kininogen Level in the Cerebrospinal Fluid May Be a Potential Biomarker for Predicting Epileptogenesis

Frontiers in Neurology
Jing ZouQinchi Lu

Abstract

Purpose: Epilepsy is a highly disabling neurological disorder. Brain insult is the most critical cause of epilepsy in adults. This study aimed to find reliable and efficient biomarkers for predicting secondary epilepsy. Materials and methods: The LiCl-pilocarpine (LiCl-Pilo) chronic epilepsy rat model was used, and rat cerebrospinal fluid (CSF) was collected 5 days after status epilepticus (SE). The CSF was analyzed using the label-free LC-ESI-Q-TOF-MS/MS. Differential expression of proteins was confirmed using enzyme-linked immunosorbent assay (ELISA) and Western blotting. The corresponding protein level in the CSF of patients with encephalitis in the postacute phase was determined using ELISA and compared between patients with and without symptomatic epilepsy after encephalitis during a 2-year follow-up. Results: The proteomics and ELISA results showed that the protein level of kininogen (KNG) was obviously elevated in both CSF and hippocampus, but not in serum, 5 days after the onset of SE in LiCl-Pilo chronic epilepsy model rats. In patients with encephalitis, the protein level of KNG in the CSF in the postacute phase was significantly elevated in patients with a recurrent epileptic seizure during a 2-year follow-up than in...Continue Reading

References

Nov 14, 2001·Neurology·M P JacobsW H Theodore
Sep 26, 2003·The New England Journal of Medicine·Bernard S Chang, Daniel H Lowenstein
Jun 16, 2004·Brain Research·Gustavo Adolfo ArgañarazJosé Antônio Silva
Feb 16, 2005·Archives of Neurology·Yang TangFrank R Sharp
Nov 10, 2006·Hippocampus·Sandra Regina PerosaMaria da Graça Naffah Mazzacoratti
Jul 25, 2007·Epilepsia·Nicola MarchiDamir Janigro
Jan 10, 2008·International Immunopharmacology·J A SilvaJ B Pesquero
Mar 11, 2008·Neuroscience Letters·Masaoki TakanoHiroshi Okamoto
Jun 14, 2008·Journal of Neuroscience Methods·Giulia CuriaMassimo Avoli
Nov 18, 2008·Neurobiology of Disease·Nicola MarchiDamir Janigro
Mar 26, 2009·Epilepsia·Melinda S KelleyUNKNOWN NINDS Epilepsy Benchmark Stewards
Apr 7, 2009·The Journal of Surgical Research·Po-Chou LiliangJiin-Haur Chuang
Sep 3, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Da-Zhi LiuFrank R Sharp
Jan 28, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Liang WangXuefeng Wang
Feb 5, 2010·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Pramod K DashAnthony N Moore
Feb 18, 2010·Future Neurology·Michael SeidenbergBruce Hermann
Oct 19, 2010·The Lancet Infectious Diseases·Julia GranerodUNKNOWN UK Health Protection Agency (HPA) Aetiology of Encephalitis Study Group
Mar 1, 2008·Proteomics. Clinical Applications·Stéphane RocheSylvain Lehmann
Jan 25, 2011·Lancet Neurology·Asla Pitkänen, Katarzyna Lukasiuk
Sep 9, 2011·The New England Journal of Medicine·Patrick KwanMartin J Brodie
Oct 19, 2011·Biomarkers in Medicine·Jerome Engel
Apr 24, 2012·Neuropharmacology·Annamaria VezzaniRaymond J Dingledine
Dec 20, 2012·Advances and Technical Standards in Neurosurgery·Hana KubovaAsla Pitkänen
Jan 23, 2014·Journal of Alzheimer's Disease : JAD·Maria Inês RosaBruno Rosa Silva
Jun 13, 2014·World Journal of Biological Chemistry·Maria da Graça Naffah-MazzacorattiSandra Regina Perosa
Jul 30, 2016·Medicine·Romain SonnevilleJean-François Timsit

❮ Previous
Next ❯

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
protein assay
electrophoresis

Software Mentioned

PEAKS Studio
ImageJ
PEAKS Q
Mascot
PEAKS

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.

Related Papers

Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
Xuan ShiDan Liu
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences
Xiangchang ZengDongsheng Ouyang
Scientific Reports
Antonella Marino GammazzaAlberto J L Macario
© 2022 Meta ULC. All rights reserved