KiSS-1 and GPR54 genes are co-expressed in rat gonadotrophs and differentially regulated in vivo by oestradiol and gonadotrophin-releasing hormone

Journal of Neuroendocrinology
N RichardM-L Kottler

Abstract

Kisspeptin, the product derived from KiSS-1, and its cognate receptor, GPR54, both exert a role in the neuroendocrine control of reproduction by regulating gonadotrophin-releasing hormone (GnRH) secretion. In the present study, we demonstrate, using dual immunofluorescence with specific antibodies, that the KiSS-1 and GPR54 genes are both expressed in rat gonadotrophs. All luteinising hormone beta-immunoreactive (LH beta-ir) cells were stained by the KiSS-1 antibody but some kisspeptin-ir cells were not LH beta positive; thus, we cannot exclude the possibility that kisspeptins are expressed in other pituitary cells. All GPR54-ir are co-localised with LH beta cells, but only a subset of LH beta cells are stained with the GPR54 antibody. Using the real-time reverse transcription-polymerase chain reaction (RT-PCR), we found that the expression of KiSS-1 and GPR54 is differentially regulated by steroids. In the female, KiSS-1 mRNA levels dramatically decreased following ovariectomy (OVX), and this decrease was prevented by administration of 17beta-oestradiol (E(2)), but not by administration of GnRH antagonist or agonist. Administration of E(2) in OVX rats receiving either GnRH antagonist or agonist clearly shows that E(2) acts dir...Continue Reading

References

Dec 4, 1996·Journal of the National Cancer Institute·J H LeeD R Welch
Jun 2, 2001·The Journal of Biological Chemistry·A I MuirD C Harrison
Aug 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nicolas de RouxEdwin Milgrom
Oct 24, 2003·The New England Journal of Medicine·Stephanie B SeminaraWilliam H Colledge
Dec 4, 2003·Biochemical and Biophysical Research Communications·Sandrine FunesEric L Gustafson
Mar 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Judit E HorvathKate Groot
Jun 16, 2004·Progress in Nucleic Acid Research and Molecular Biology·Stephen Safe, Kyounghyun Kim
Jun 29, 2004·Biochemical and Biophysical Research Communications·Hisanori MatsuiTetsuya Ohtaki
Feb 3, 2005·Proceedings of the National Academy of Sciences of the United States of America·Muhammad ShahabTony M Plant
May 28, 2005·Endocrinology·Jeremy T SmithRobert A Steiner
May 9, 2006·Cell·David M Lonard, Bert W O'Malley
Jul 11, 2006·Endocrinology·J M CastellanoM Tena-Sempere
Dec 14, 2006·The Journal of Clinical Endocrinology and Metabolism·Yardena Tenenbaum-RakoverNicolas de Roux

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Citations

Aug 18, 2012·Archives of Gynecology and Obstetrics·Heather CockwellMichael Wilkinson
Mar 10, 2011·Endocrine·Yathindar S RaoToni R Pak
Dec 26, 2008·Human Reproduction Update·A K Roseweir, R P Millar
Nov 1, 2009·Integrative and Comparative Biology·Wilbur P WilliamsLance J Kriegsfeld
Mar 25, 2010·Neuroendocrinology·Paul MicevychJohn Kuo
Sep 23, 2009·Endocrine Reviews·Amy E OakleyRobert A Steiner
Dec 24, 2011·European Journal of Histochemistry : EJH·A PorzionatoR De Caro
Sep 16, 2015·International Journal of Environmental Research and Public Health·Xiaona HuoJun Zhang
May 15, 2013·Theriogenology·Eleni DovolouGeorgios S Amiridis
Aug 21, 2012·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Hirofumi HashimotoYoichi Ueta
Feb 16, 2010·Biochemical and Biophysical Research Communications·Mia C DeFinoChris Hague
Apr 2, 2016·Research in Veterinary Science·Paulina Radwańska, Urszula Kosior-Korzecka
Oct 25, 2008·Peptides·Nicolas RichardMarie-Laure Kottler
Jul 23, 2008·Peptides·Yee-Ming ChanStephanie B Seminara
Jul 23, 2008·Peptides·Michelle L GottschRobert A Steiner
Jun 16, 2015·Peptides·Onder CelikMusa Yilmaz
Feb 12, 2009·Journal of Neuroendocrinology·J Clarkson, A E Herbison
Oct 11, 2013·The Journal of Reproduction and Development·Yousuke NaniwaSatoshi Ohkura
Jul 25, 2013·Molecular and Cellular Endocrinology·Tomohiro TerasakaHirofumi Makino
Jun 29, 2016·Theriogenology·Briony M McGrathScott T Norman
Aug 2, 2016·Molecular and Cellular Endocrinology·Manuel D GaheteRaúl M Luque
Jun 17, 2011·Biology of Reproduction·Juan RoaManuel Tena-Sempere
Mar 20, 2018·The Journal of Endocrinology·J T SmithR P Millar
May 31, 2008·Journal of Neuroendocrinology·W S Dhillo
Mar 13, 2014·Human Reproduction Update·Karolina SkorupskaiteRichard A Anderson

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