KISS-1 inhibits the proliferation and invasion of gastric carcinoma cells.

World Journal of Gastroenterology : WJG
Na LiGuo-Yang He

Abstract

To investigate the function of the KISS-1 gene in gastric carcinoma cells and to explore its potential mechanism. A KISS-1 eukaryotic expression vector was constructed and transfected into BGC-823 cells. Resistant clones were obtained through G418 selection. reverse transcription-polymerase chain reaction and western blotting were used to detect KISS-1 and matrix metalloproteinase-9 (MMP-9) expression in transfected cells. The growth of transfected cells was investigated by 3-(4, 5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) proliferation assays, and the cells' invasive potential was analyzed by basement membrane (Matrigel) invasion assays. The anti-tumor effects of KISS-1 were tested in vivo using allografts in nude mice. The expression level of KISS-1 mRNA and protein in BGC-823/KISS-1 transfected cells were significantly higher than in BGC-823/pcDNA3.1 transfected cells (P < 0.05) or the parental BGC-823 cell line (P < 0.05). The expression level of MMP-9 mRNA and protein in BGC-823/KISS-1 were significantly less than in BGC-823/pcDNA3.1 (P < 0.05) or BGC-823 cells (P < 0.05). MTT growth assays show the proliferation of BGC-823/KISS-1 cells at 48 h (0.642 ± 0.130) and 72 h (0.530 ± 0.164) were significantly re...Continue Reading

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Citations

Jul 15, 2015·Pathology Oncology Research : POR·Lucheng ZhuShirong Zhang
Apr 4, 2013·Critical Reviews in Biochemistry and Molecular Biology·Jennifer VandoorenGhislain Opdenakker
Apr 4, 2018·PloS One·Xiaoyang ZhangZhi-Liang Lu
May 26, 2016·Asian Pacific Journal of Cancer Prevention : APJCP·Richa SinghRajendra Kumar
May 16, 2018·Frontiers in Endocrinology·Federica FratangeloMaria Letizia Motti

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