Kiwifruit L-galactose dehydrogenase: molecular, biochemical and physiological aspects of the enzyme

Functional Plant Biology : FPB
William A LaingElspeth MacRae

Abstract

l-Galactose dehydrogenase, an enzyme in the pathway of ascorbate biosynthesis, was purified to homogeneity from leaves of kiwifruit [Actinidia deliciosa (A.Chev.) CF Liang et AR Ferguson var. deliciosa 'Hayward']. The enzyme had a molecular mass of 34.2 kD, and behaved as a monomer during gel filtration. The Km(galactose) and Km(NAD) decreased as pH increased from 6.5 to 9, while the Vmax increased over this range. A number of related sugars were tested as alternative substrates or inhibitors, but these were ineffective. Ascorbate caused slow inactivation of the enzyme, possibly through metal catalysed generation of oxygen radicals. Inactivation appeared to be active-site directed as it was protected by the substrate NAD, and not by NADH or l-galactose. This is not likely to be physiologically significant. Through partially sequencing the protein, the gene was identified in the HortResearch Actinidia EST database, and the translation of the full length sequence of this cDNA showed a high homology (80% identity, 90% similarity) to the translation of an Arabidopsis gene (accession CAD10386) and to translations of other genes identified in GenBank. Levels of l-GalDH activity decreased during fruit and leaf development, and levels ...Continue Reading

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Citations

Dec 1, 2014·Botanical Studies·Jelli Venkatesh, Se Won Park
May 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·William A LaingSean M Bulley
May 1, 2009·Functional Plant Biology : FPB·Ken B MarshWilliam A Laing
Nov 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·William A LaingElspeth MacRae
Feb 9, 2019·Frontiers in Plant Science·Mario FenechMiguel A Botella
Apr 2, 2021·Plant Physiology·Mario FenechMiguel A Botella

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