KLC3 is involved in sperm tail midpiece formation and sperm function

Developmental Biology
Ying ZhangFrans A van der Hoorn

Abstract

Kinesin light chain 3 (KLC3) is the only known kinesin light chain expressed in post-meiotic male germ cells. We have reported that in rat spermatids KLC3 associates with outer dense fibers and mitochondrial sheath. KLC3 is able to bind to mitochondria in vitro and in vivo employing the conserved tetratrico-peptide repeat kinesin light chain motif. The temporal expression and association of KLC3 with mitochondria coincides with the stage in spermatogenesis when mitochondria move from the spermatid cell periphery to the developing midpiece suggesting a role in midpiece formation. In fibroblasts, expression of KLC3 results in formation of large KLC3 aggregates close to the nucleus that contain mitochondria. However, the molecular basis of the aggregation of mitochondria by KLC3 and its role in sperm tail midpiece formation are not clear. Here we show that KLC3 expression from an inducible system causes mitochondrial aggregation within 6h in a microtubule dependent manner. We identified the mitochondrial outer membrane porin protein VDAC2 as a KLC3 binding partner. To analyze a role for KLC3 in spermatids we developed a transgenic mouse model in which a KLC3ΔHR mutant protein is specifically expressed in spermatids: this KLC3 muta...Continue Reading

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Citations

Aug 1, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Alexandra AmaralJoão Ramalho-Santos
Jul 9, 2013·Molecular and Cellular Endocrinology·Mari S LehtiAnu Sironen
Oct 13, 2017·Biology of Reproduction·Mari S Lehti, Anu Sironen
Dec 23, 2016·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hidenobu OkudaMoira K O'Bryan
Apr 23, 2020·Human Reproduction Update·Christiane PleugerMoira K O'Bryan
Dec 26, 2018·Parasites & Vectors·Wanqi Hu, Zhijian Jake Tu
Sep 10, 2020·Biology·Alexandra Almansa-OrdonezFilippo Zambelli
Jun 14, 2020·Mitochondrion·Singh VertikaSingh Rajender
Nov 26, 2020·Biology of Reproduction·Naina Kumar, Amit Kant Singh
Jun 2, 2021·Open Biology·Bárbara MatosMargarida Fardilha
Nov 6, 2021·Proceedings of the National Academy of Sciences of the United States of America·Miguel Ricardo LeungTzviya Zeev-Ben-Mordehai

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