Abstract
A novel Gram-negative, aerobic, rod-shaped and pesticide (chlorpyrifos and atrazine) tolerant strain, designated CW-D 3(T), was isolated from a heavily polluted soil sample in Jiangsu Province, China. The strain could tolerate about 800 microg.ml(-1) atrazine or chlorpyrifos when added in TYB medium. A polyphasic taxonomy including phylogenetic and phenotypic analysis was performed on the new isolate. Phylogenetic analysis and chemotaxonomic characteristics revealed that strain CW-D 3(T) belongs to the genus Klebsiella. Its closest phylogenetic neighbors were K. singaporensis (strain LX3(T)), three subspecies of K. pneumoniae (strains ATCC 13883(T), ATCC 13884(T) and ATCC 11296(T)) and K. granulomatis, according to 16S rRNA and rpoB gene analysis. The 16S rRNA gene sequence similarity was about 94.8-97.6% and the rpoB gene sequence similarity was about 96.8-98.6% to its phylogenetic neighbors. Strain CW-D 3(T) also showed low DNA-DNA reassociation values (less than 21%) with respect to the three subspecies of K. pneumoniae and Klebsiella singaporensis. Its major fatty acids were C14:0, C16:0, c-C17:0 and c-C19:0 omega8c. The G+C content of the DNA was 58.3 mol%. Phylogenetic and phenotypic results supported the assignment of CW...Continue Reading
References
Apr 1, 1992·International Journal of Systematic Bacteriology·G SpieringsJ Tommassen
Aug 1, 1985·Journal of Clinical Pathology·R J WigginsS Tabaqchali
Jan 1, 1971·Advances in Genetics·D J Brenner, S Falkow
Jan 1, 1970·European Journal of Biochemistry·J De LeyA Reynaerts
Dec 1, 1980·Journal of Molecular Evolution·M Kimura
Feb 28, 1998·Nucleic Acids Research·J D ThompsonD G Higgins
Nov 26, 1998·International Journal of Systematic Bacteriology·N V DoroninaT P Tourova
Nov 11, 1999·International Journal of Systematic Bacteriology·J S CarterD J Kemp
Aug 5, 2000·Applied and Environmental Microbiology·S Yamaguchi, M Yokoe
Jun 20, 2001·International Journal of Systematic and Evolutionary Microbiology·M DrancourtP Rousselier
Jul 21, 2004·Briefings in Bioinformatics·Sudhir KumarMasatoshi Nei
Nov 17, 2004·International Journal of Systematic and Evolutionary Microbiology·Xianzhen LiLianhui Zhang
Jul 13, 2007·International Journal of Systematic and Evolutionary Microbiology·Wen-Jun LiCheng-Lin Jiang
Aug 9, 2007·International Journal of Systematic and Evolutionary Microbiology·Yu ZhouWen-Jun Li
Jun 3, 2008·Rheumatology·N HunzelmannUNKNOWN Registry of the German Network for Systemic Scleroderma
Mar 14, 2009·The Journal of General and Applied Microbiology·Yu ZhouRen Lai
Jan 1, 1983·Systematic and Applied Microbiology·V A HussK H Schleifer
Citations
May 21, 2011·Annals of Clinical Microbiology and Antimicrobials·Arvind KumarRanadhir Chakraborty
Apr 18, 2013·PloS One·Eugenijus SimoliūnasRolandas Meškys
Apr 1, 2016·International Journal of Systematic and Evolutionary Microbiology·Chun Yan LiChun Tao Gu
Jul 21, 2016·Diagnostic Microbiology and Infectious Disease·J Michael Janda
Sep 28, 2018·Journal of Clinical Microbiology·Erik Munson, Karen C Carroll
Apr 12, 2014·The Journal of Microbiology·Dan WangXiaohui Zhang
May 8, 2020·International Journal of Systematic and Evolutionary Microbiology·Sukriti GujaratiPraveen Rahi
Nov 8, 2016·Canadian Journal of Microbiology·Yu-Jie ZhouShu-Lin Liu
Nov 1, 2015·International Journal of Systematic and Evolutionary Microbiology·Aharon Oren, George M Garrity
Jun 9, 2021·Journal of Basic Microbiology·Zhen WangXiang Li