PMID: 3322266Nov 1, 1987Paper

Klebsiella pneumoniae nitrogenase. Inhibition of hydrogen evolution by ethylene and the reduction of ethylene to ethane

The Biochemical Journal
G A AshbyR N Thorneley

Abstract

Ethylene (C2H4) inhibited H2 evolution by the Mo-containing nitrogenase of Klebsiella pneumoniae. The extent of inhibition depended on the electron flux determined by the ratio of Fe protein (Kp2) to MoFe protein (Kp1) with KiC2H4 = 409 kPa ([Kp2]/[Kp1] = 22:1) and KC2H4i = 88 kPa ([Kp1]/[Kp2] = 21:1) at 23 degrees C at pH 7.4. At [Kp2]/[Kp1] = 1:1, inhibition was minimal with C2H4 (101 kPa). Extrapolation of data obtained when C2H4 was varied from 60 to 290 kPa indicates that at infinite pressure of C2H4 total inhibition of H2 evolution should occur. C2H4 inhibited concomitant S2O4(2-) oxidation to the same extent that it inhibited H2 evolution. Although other inhibitors of total electron flux such as CN- and CH3NC uncouple MgATP hydrolysis from electron transfer, C2H4 did not affect the ATP/2e ratio. Inhibition of H2 evolution by C2H4 was not relieved by CO. C2H4 was reduced to C2H6 at [Kp2]/[Kp1] ratios greater than or equal to 5:1 in a reaction that accounted for no more than 1% of the total electron flux. These data are discussed in terms of the chemistry of alkyne and alkene reduction on transition-metal centres.

Citations

Apr 20, 2013·Biochimica Et Biophysica Acta·Lance C SeefeldtDennis R Dean
Jan 15, 2008·Journal of Industrial Microbiology & Biotechnology·Vipin Chandra Kalia, Hemant J Purohit
May 12, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Zofia MaskosBrian J Hales
Jun 24, 2014·Dalton Transactions : an International Journal of Inorganic Chemistry·Zhenyu ZuoXiaoyan Li
Mar 17, 2020·Chemical Reviews·Lance C SeefeldtBrian M Hoffman
Nov 7, 1996·Chemical Reviews·Barbara K. Burgess, David J. Lowe

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