PMID: 701786Oct 1, 1978Paper

Klebsiella serotyping by counter-current immunoelectrophoresis

The Journal of Hygiene
J M Palfreyman

Abstract

The development of a Klebsiella serotyping method by counter-current immunoelectrophoresis (CIE) is described. Antisera were prepared against the capsular antigens of 72 type strains and tested for the specificity and strength of their precipitin reactions with antigens from homologous and heterologous serotypes. All antisera produced strong reactions with their homologous antigen: when diluted to titre 63 were highly specific, 3 cross-reacted strongly and 6 weakly with one other antigen. Pools of antisera for screening purposes were constructed on the basis of common crossreactions: component serotypes of each pool could be detected strongly and specifically. The technique is simple to perform, fairly rapid, and economical in the use of antisera. Results can be read easily and quickly and the intensity of cross-reactions compared directly. The technique appears to be more specific and is less time consuming than the Quellung method, but further assessment of its efficacy in typing routine clinical cultures is necessary.

References

Aug 27, 1977·Lancet·M W CasewellI Phillips
Apr 1, 1976·Journal of Clinical Pathology·E RiserT A Poulton
Apr 1, 1976·Journal of Clinical Pathology·E RiserM L Bonnet
May 11, 1975·The Journal of Infectious Diseases·E R NoriegaJ J Rahal
Oct 17, 1970·Lancet·J Z MontgomerieW J Martin
May 1, 1971·Annals of Internal Medicine·R SeldenT C Eickhoff

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Citations

Mar 24, 2000·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·D E RobertsE W Howerth
Oct 4, 2013·Journal of Clinical Microbiology·Sylvain BrisseDominique Decré
Jul 20, 2018·Journal of Fluorescence·Sepideh AhmadiMohammad Reza Sharifmoghadam
Jun 1, 1979·The Journal of Applied Bacteriology·A S Edmondson, E M Cooke
Oct 1, 1984·The Journal of Hygiene·E A Roe, R J Jones
May 1, 1994·The Journal of Applied Bacteriology·Y MengistuJ R Saunders
Jan 25, 2008·Emerging Infectious Diseases·Victor L YuUNKNOWN International Klebseilla Study Group
Oct 1, 1985·The Journal of Hygiene·A M Simoons-SmitD M MacLaren
May 28, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Y-T LinC-P Fung
Jul 23, 2015·MBio·Carsten StruveKaren A Krogfelt
Feb 1, 1985·Infection Control : IC·S J Rubin
Feb 13, 2001·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·I SechterD S Hansen
May 30, 2008·Journal of Clinical Microbiology·Yi-Jiun PanJin-Town Wang
Feb 1, 1997·Journal of Clinical Microbiology·M GazouliL S Tzouvelekis
Apr 1, 1986·Journal of Clinical Microbiology·S J CryzR Germanier
Jul 1, 1981·Journal of Clinical Microbiology·A BauernfeindR Schneider
Mar 1, 1989·Journal of Clinical Microbiology·M A GastonT L Pitt
Jul 1, 1989·Journal of Clinical Microbiology·J Z MontgomerieE M Jimenez
Aug 1, 1996·The Journal of Hospital Infection·R P HobsonI M Gould
Aug 30, 2002·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·D S HansenH J Kolmos

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