Klf2 and Tfcp2l1, Two Wnt/β-Catenin Targets, Act Synergistically to Induce and Maintain Naive Pluripotency

Stem Cell Reports
Dongbo QiuQi-Long Ying

Abstract

Activation of Wnt/β-catenin signaling can induce both self-renewal and differentiation in naive pluripotent embryonic stem cells (ESCs). To gain insights into the mechanism by which Wnt/β-catenin regulates ESC fate, we screened and characterized its downstream targets. Here, we show that the self-renewal-promoting effect of Wnt/β-catenin signaling is mainly mediated by two of its downstream targets, Klf2 and Tfcp2l1. Forced expression of Klf2 and Tfcp2l1 can not only induce reprogramming of primed state pluripotency into naive state ESCs, but also is sufficient to maintain the naive pluripotent state of ESCs. Conversely, downregulation of Klf2 and Tfcp2l1 impairs ESC self-renewal mediated by Wnt/β-catenin signaling. Our study therefore establishes the pivotal role of Klf2 and Tfcp2l1 in mediating ESC self-renewal promoted by Wnt/β-catenin signaling.

References

Dec 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·G R Martin
Feb 12, 2008·Nature Cell Biology·Jianming JiangHuck-Hui Ng
Mar 1, 2012·Nature Biotechnology·Thomas O'LearyPetra De Sutter
Jun 7, 2014·Cell Stem Cell·Jia-Chi YeoHuck-Hui Ng

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Citations

Mar 24, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Daniela Ávila-GonzálezNéstor Fabián Díaz
Jan 4, 2017·Nature Reviews. Genetics·Mo Li, Juan Carlos Izpisua Belmonte
Jun 21, 2017·Biochemical and Biophysical Research Communications·Kuisheng LiuShoudong Ye
Feb 24, 2018·The Journal of Experimental Medicine·Stefanie SchmidtStefan Rose-John
Jan 13, 2018·Journal of Cellular Physiology·Hongwei SunShoudong Ye
Mar 8, 2018·American Journal of Physiology. Cell Physiology·Xiaojuan XuShoudong Ye
Nov 30, 2018·The EMBO Journal·Sara-Jane DunnGraziano Martello
Jan 28, 2020·Reproduction in Domestic Animals = Zuchthygiene·Haijing ZhuJinlian Hua
Feb 9, 2019·Nature Communications·J MathieuH Ruohola-Baker
May 26, 2017·Stem Cells and Development·Ludovic ZimmerlinElias T Zambidis
May 4, 2018·Nature·Nicolas C RivronNiels Geijsen
Apr 28, 2017·Molecular Medicine Reports·Wiktoria Maria SuchorskaTomasz Trzeciak
Mar 16, 2017·Biology Open·Shoudong YeQi-Long Ying
Aug 24, 2021·Frontiers in Cell and Developmental Biology·Mengyi WeiSiqin Bao

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Datasets Mentioned

BETA
GSE31461
GSE46369
GSE50393

Methods Mentioned

BETA
immunoprecipitation

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