Abstract
Krüppel-like factor 4 (KLF4) is an evolutionarily conserved zinc finger-containing transcription factor with diverse regulatory functions in cell growth, proliferation, differentiation, and embryogenesis. In our previous study, we found that KLF4 mRNA was up-regulated more than 10-fold in adult mice lung tissues after endotoxin stimuli, and that KLF4 can regulate the expression of IL -10, an early inflammatory mediator. To determine whether KLF4 influences the expression and release of high-mobility group box 1 (HMGB1), an important late inflammatory mediator, which contains two potential KLF4-binding elements in its promoter, pcDNA3.1-KLF4 expression plasmid or KLF4 antisense oligonucleotide was transfected into RAW264.7 macrophages, the expression and release of HMGB1 were examined by reverse-transcriptase-polymerase chain reaction and Western blot, respectively. Electrophoretic mobility shift assay was performed to detect the binding activity of KLF4 to the HMGB1 promoter. The results showed that KLF4 overexpression led to an increased expression of HMGB1 in both cytoplasm and nucleus, whereas KLF4 deficiency led to a decrease in HMGB1. Moreover, compared with the control group, the release of HMGB1 was increased after KLF4 ...Continue Reading
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