Klhl31 attenuates β-catenin dependent Wnt signaling and regulates embryo myogenesis

Developmental Biology
Alaa Abou-ElhamdAndrea Münsterberg

Abstract

Klhl31 is a member of the Kelch-like family in vertebrates, which are characterized by an amino-terminal broad complex tram-track, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domain, carboxy-terminal Kelch repeats and a central linker region (Back domain). In developing somites Klhl31 is highly expressed in the myotome downstream of myogenic regulators (MRF), and it remains expressed in differentiated skeletal muscle. In vivo gain- and loss-of-function approaches in chick embryos reveal a role of Klhl31 in skeletal myogenesis. Targeted mis-expression of Klhl31 led to a reduced size of dermomyotome and myotome as indicated by detection of relevant myogenic markers, Pax3, Myf5, myogenin and myosin heavy chain (MF20). The knock-down of Klhl31 in developing somites, using antisense morpholinos (MO), led to an expansion of Pax3, Myf5, MyoD and myogenin expression domains and an increase in the number of mitotic cells in the dermomyotome and myotome. The mechanism underlying this phenotype was examined using complementary approaches, which show that Klhl31 interferes with β-catenin dependent Wnt signaling. Klhl31 reduced the Wnt-mediated activation of a luciferase reporter in cultured cells. Furthermore, Klhl31 attenuated secondar...Continue Reading

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Citations

Feb 9, 2016·Seminars in Cell & Developmental Biology·Armbien SabilloCarmen R Domingo
May 29, 2018·Development·Gi Fay MokAndrea Münsterberg
Sep 6, 2017·The Journal of Clinical Investigation·James B PapizanEric N Olson
Jun 2, 2018·International Journal of Cell Biology·Vincenzo GiancottiClaudio Rizzi
Jan 19, 2021·Gene Expression Patterns : GEP·Abdulmajeed Fahad AlrefaeiGrant N Wheeler
May 3, 2021·Biochemical and Biophysical Research Communications·Genghua ChenWen Luo

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