Klotho inhibits growth and promotes apoptosis in human lung cancer cell line A549.

Journal of Experimental & Clinical Cancer Research : CR
Bo ChenJianqing Wu

Abstract

Klotho, as a new anti-aging gene, can shed into circulation and act as a multi-functional humoral factor that influences multiple biological processes. Recently, published studies suggest that klotho can also serve as a potential tumor suppressor. The aim of this study is to investigate the effects and possible mechanisms of action of klotho in human lung cancer cell line A549. In this study, plasmids encoding klotho or klotho specific shRNAs were constructed to overexpress or knockdown klotho in vitro. A549 cells were respectively treated with pCMV6-MYC-KL or klotho specific shRNAs. The MTT assay was used to evaluate the cytotoxic effects of klotho and flow cytometry was utilized to observe and detect the apoptosis of A549 cells induced by klotho. The activation of IGF-1/insulin signal pathways in A549 cells treated by pCMV6-MYC-KL or shRNAs were evaluated by western blotting. The expression levels of bcl-2 and bax transcripts were evaluated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Overexpression of klotho reduced the proliferation of lung cancer A549 cells, whereas klotho silencing in A549 cells enhanced proliferation. Klotho did not show any effects on HEK-293 cells. Klotho overexpression in...Continue Reading

References

Nov 15, 1996·Cell·T F Gajewski, C B Thompson
Feb 17, 1998·Biochemical and Biophysical Research Communications·Y MatsumuraY Nabeshima
Jan 29, 1999·Journal of the National Cancer Institute·H YuX Wu
Oct 12, 2000·Biochemical and Biophysical Research Communications·Y SaitoR Nagai
Oct 26, 2000·Nature·M O Hengartner
Apr 23, 2002·Hypertension·Haruo MitaniRyozo Nagai
Feb 5, 2003·Biochemical and Biophysical Research Communications·Jin YangToshio Ogihara
Mar 1, 2003·Science·Marc TatarAdam Antebi
Aug 2, 2005·Drug Discovery Today·Francesco Hofmann, Carlos García-Echeverría
Aug 27, 2005·Science·Hiroshi KurosuMakoto Kuro-o
Aug 11, 2007·Science·Hongjun LiuToren Finkel
Jul 25, 2008·Nature·Kezhong Zhang, Randal J Kaufman
May 29, 2009·CA: a Cancer Journal for Clinicians·Ahmedin JemalMichael J Thun
Aug 19, 2009·Journal of Cellular Physiology·Stefano MattarocciMarco G Paggi

❮ Previous
Next ❯

Citations

Nov 2, 2011·Breast Cancer Research and Treatment·Tami RubinekIdo Wolf
Jul 6, 2013·Pathology Oncology Research : POR·Biao XieJunkun Zhan
Apr 28, 2011·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jie PanLiang Jing Wang
Apr 13, 2011·PloS One·Apostolos ZaravinosDemetrios A Spandidos
Oct 8, 2013·The Journal of Endocrinology·Christoph SchmidRené L Bernays
Jul 3, 2013·Age·Stephen J MehiGwendalyn D King
Jun 1, 2014·Bone·Despoina GkentziDionisios Chrysis
Jul 26, 2015·Journal of Clinical Pathology·Sarit Aviel-RonenIris Barshack
Sep 19, 2012·Cell Biochemistry and Function·Alana de VasconcelosAlethéa Gatto Barschak
Sep 24, 2011·The Biochemical Journal·Gwendalyn D KingCarmela R Abraham
Aug 5, 2014·Journal of Cancer Research and Clinical Oncology·Xiangxiang Zhou, Xin Wang
Jun 5, 2014·Archives of Toxicology·In-Sik ShinMee-Young Lee
Mar 18, 2017·Medical Molecular Morphology·Bhoj Raj AdhikariYoshihiro Abiko
Dec 19, 2017·Frontiers in Endocrinology·George D DaltonChou-Long Huang
Feb 20, 2015·Endocrine Reviews·Yuechi Xu, Zhongjie Sun
Sep 6, 2017·Respiratory Research·Judit RappJudit E Pongracz
May 8, 2018·Oncotarget·Shunta HoriKiyohide Fujimoto
May 28, 2019·Journal of Cellular Biochemistry·Maryam AbolghasemiDurdi Qujeq
Aug 31, 2016·Oncology Reports·Shunta HoriKiyohide Fujimoto
Jun 28, 2018·Pathology Oncology Research : POR·Judit PakoIldiko Horvath
Sep 25, 2019·International Journal of Molecular Sciences·Kenneth LimIrene Chong
Jun 27, 2020·Cancers·Aishani SachdevaIrene Chong
May 17, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Lilach AbramovitzIdo Wolf
Jun 27, 2015·Molecular Cancer Research : MCR·Hagai LigumskyIdo Wolf
Mar 4, 2020·FASEB BioAdvances·Jianning ZhangConnie C W Hsia

❮ Previous
Next ❯

Methods Mentioned

BETA
transfections
ELISA
transfection
flow cytometry

Software Mentioned

SPSS
GraphPad Prism
GraphPad

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis