KmrA multidrug efflux pump from Klebsiella pneumoniae

Biological & Pharmaceutical Bulletin
Wakano OgawaTomofusa Tsuchiya

Abstract

We cloned a gene responsible for multidrug resistance from the chromosomal DNA of Klebsiella pneumoniae MGH78578 that showed multidrug resistance. We designated the gene kmrA. The deduced amino acid sequence of KmrA was similar to that of SmvA that is responsible for methyl viologen-resistance in Salmonella enterica sv. Typhi and Typhimurium. Introduction of the cloned kmrA gene into drug-hypersensitive Escherichia coli KAM32 cells made them resistant to acriflavine, 4',6-diamidino-2-phenylindole (DAPI), Hoechst 33342, tetraphenylphosphonium chloride (TPPCl), methyl viologen and ethidium bromide. We observed elevated energy-dependent efflux of ethidium in E. coli cells carrying the kmrA gene compared with control cells. We also cloned the smvA gene from S. enterica sv. Typhimurium LT2 and investigated the resistance pattern for several drugs. The pattern was similar between KmrA from K. pneumoniae and SmvA from S. enterica.

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Citations

May 16, 2014·PloS One·Vijaya Bharathi SrinivasanGovindan Rajamohan
Mar 29, 2014·Nature Communications·Thomas L RueggMichael P Thelen
May 20, 2009·Antimicrobial Agents and Chemotherapy·G E LouwT C Victor
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Apr 6, 2007·Biological & Pharmaceutical Bulletin·Fereshteh ShahcheraghiTomofusa Tsuchiya
Apr 2, 2008·Biological & Pharmaceutical Bulletin·Dai-Wei LiTomofusa Tsuchiya
Dec 2, 2008·Biochimica Et Biophysica Acta·Helen I ZgurskayaGanesh Krishnamoorthy
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Aug 16, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Lucia PulzovaLubos Comor
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Jan 1, 2013·International Journal of Bacteriology·Sanath KumarManuel F Varela

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