PMID: 8599613Feb 29, 1996Paper

KN-62, a calcium/calmodulin-dependent protein kinase II inhibitor, inhibits high potassium-stimulated prolactin secretion and intracellular calcium increases in anterior pituitary cells

Biochimica Et Biophysica Acta
Z J CuiP S Dannies

Abstract

In isolated rat anterior pituitary cells, KN-62 (10 microM), an isoquinoline sulfonamide inhibitor of calcium/calmodulin-dependent protein kinase II, inhibited high KCl(50 milliM)-stimulated prolactin secretion almost completely, with an IC50 of 95 nM KN-62 inhibited TRH-induced prolactin secretion less effectively. KN-04, a compound that is over 100-fold less active in inhibiting purified calcium/calmodulin-dependent protein kinase II, also inhibited high KCl-stimulated prolactin secretion with an IC50 of 500 nM. KN-62 and KN-04 (10 microM) both inhibited high KCl-stimulated increases in intracellular Ca2+ concentrations. We conclude that KN-62 and KN-04 inhibit activation of voltage-dependent calcium channels in anterior pituitary cells either directly or indirectly.

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Citations

Nov 14, 1997·Brain Research. Developmental Brain Research·G AudesirkM Kern
Mar 15, 2011·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Carlo NapolitanoSilvia G Priori
Jan 1, 2010·Hormone Molecular Biology and Clinical Investigation·Marko A PopovicArturo E Gonzalez-Iglesias

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