Knitted 3D Scaffolds of Polybutylene Succinate Support Human Mesenchymal Stem Cell Growth and Osteogenesis

Stem Cells International
Miina OjansivuSusanna Miettinen

Abstract

Polybutylene succinate (PBS) is a biodegradable polyester with better processability and different mechanical properties compared to polylactides (PLAs), the most commonly used synthetic polymers in tissue engineering (TE). Since only few studies have evaluated PBS-containing materials for bone TE, we prepared PLA-PBS blends and analyzed material properties as well as cell attachment, proliferation, and osteogenic differentiation of human mesenchymal stem cells (hMSCs) on scaffolds. In addition to PLA, PBS, and PLA-PBS blends, PLA-polycaprolactone and PLA-poly(trimethylene carbonate) blends were evaluated. Polymer fibers were prepared using melt spinning. Pure PBS was observed to have the highest crystallinity and strain at break compared to the tougher PLA and PLA blends. No degradation occurred during the 4-week hydrolysis in either of the materials. Knitted and rolled scaffolds were manufactured, seeded with hMSCs, and cultured for 27 days. Human MSC viability was good on all the materials, but cell spreading along the fibers was only detected in PBS-containing scaffolds. They also induced the strongest proliferative response and osteogenic differentiation, which diminished with decreasing PBS content. Based on these results...Continue Reading

References

Apr 8, 2004·Developmental Cell·Rowena McBeathChristopher S Chen
May 13, 2005·Macromolecular Bioscience·Haiyan LiJunying Wang
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
Sep 25, 2008·Journal of Biomedical Materials Research. Part a·Manus J P BiggsMathew J Dalby
Mar 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kristopher A KilianMilan Mrksich
Feb 12, 2011·Journal of Tissue Engineering and Regenerative Medicine·A R Costa-PintoN M Neves
Jan 31, 2012·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Hua KunYang Xiubin
Feb 19, 2013·Stem Cell Research & Therapy·Laura KyllönenSusanna Miettinen
Jan 9, 2014·Journal of Translational Medicine·Mahmood S ChoudheryDavid T Harris
May 3, 2014·Journal of Biomedical Materials Research. Part a·Somying PatntirapongWanida Janvikul
Dec 7, 2014·BMC Biotechnology·Yuichiro YamaguchiTadao Fukushima
Aug 4, 2015·Acta Biomaterialia·Julian R Jones
Jan 29, 2016·Journal of Translational Medicine·Amy L StrongBruce A Bunnell
Jul 15, 2016·Journal of Tissue Engineering and Regenerative Medicine·Viviana P RibeiroRui L Reis
May 10, 2017·Journal of Tissue Engineering and Regenerative Medicine·Eelco M FennemaJan de Boer

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Citations

Jul 10, 2019·Stem Cells International·Vasilis Paspaliaris, George Kolios
Nov 10, 2020·Bio-medical Materials and Engineering·Mouffouk FouziSihem Aouabdi

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Methods Mentioned

BETA
differential scanning calorimetry
X-ray
Assay

Software Mentioned

CyQUANT
Avizo
SPSS Statistics

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