Knock down of Caenorhabditis elegans cutc-1 exacerbates the sensitivity toward high levels of copper

Toxicological Sciences : an Official Journal of the Society of Toxicology
Sara CalafatoStephen R Stürzenbaum

Abstract

Copper, though toxic in excess, is an essential trace element that serves as a cofactor in many critical biological processes such as respiration, iron transport, and oxidative stress protection. To maintain this balance between requirement and toxicity, biological systems have developed intricate systems allowing the preservation of homeostasis while ensuring delivery of copper to the appropriate cellular component. The nematode Caenorhabditis elegans was exploited to assess the effects of copper toxicity at the population level to identify key changes in life cycle traits including, lethality, brood size, generation time, growth, and life span. To enhance our understanding of the complexities of copper homeostasis at the genetic level, the expression profile and functional significance of a putative copper cytoplasmic metallochaperone cutc-1 were analyzed. Using quantitative PCR technology, cutc-1 was found to be downregulated with increasing CuSO(4) concentrations. However, although total (whole body) copper levels increased in nematodes exposed to elevated levels of copper, wild-type and knock down of cutc-1 by RNA-mediated interference (RNAi) were statistically indistinguishable. Nevertheless, RNAi of cutc-1 affected brood...Continue Reading

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Citations

Nov 6, 2009·The Journal of Biological Chemistry·Yan Zhang, Vadim N Gladyshev
Sep 14, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Kathleen L MorganMiguel Estevez
Sep 25, 2009·Journal of the Royal Society, Interface·Napachanok MongkoldhumrongkulStephen Stürzenbaum
Sep 20, 2011·Applied and Environmental Microbiology·Thomas MosserMagali Leroy
Dec 15, 2015·Biological Trace Element Research·Remesh KunjunniVivekanandhan Subbiah
Mar 3, 2011·Biochemical and Biophysical Research Communications·Mauricio LatorreMauricio González
Jul 10, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Piper Reid HuntRobert L Sprando
Aug 9, 2017·Environmental Toxicology and Chemistry·Sofie MoysonSteven J Husson
Nov 3, 2017·Environmental Toxicology and Chemistry·Fidelis MugovaDavid Spurgeon
Jun 9, 2020·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Xiaolu ZhangPernilla Wittung-Stafshede
May 26, 2021·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Kehinde D FasaeMichael Aschner

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