'Knock down' of DNA polymerase beta by RNA interference: recapitulation of null phenotype

DNA Repair
Yaroslava Y PolosinaHolly Miller

Abstract

DNA polymerase beta (pol beta) is the major DNA polymerase involved in the base excision repair (BER) pathway in mammalian cells and, as a consequence, BER is severely compromised in cells lacking pol beta. Pol beta null (-/-) mouse embryos are not viable and pol beta null cells are hypersensitive to alkylating agents. Using RNA interference (RNAi) technology in mouse cells, we have reduced the pol beta protein and mRNA to undetectable levels. Pol beta knockdown cell lines display a pattern of hypersensitivity to DNA damaging agents similar to that observed in pol beta null cells. Generation of pol beta knock down cells makes it possible to combine the pol beta null phenotype with deficiencies in other DNA repair proteins, thereby helping to elucidate the role of pol beta and its interactions with other proteins in mammalian cells.

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Citations

Jul 23, 2005·The Journal of Physiology·V ValiunasP R Brink
Feb 13, 2014·Journal of the American Chemical Society·Dumitru ArianMarc M Greenberg
Aug 19, 2008·Biochimie·Yukinobu UchiyamaKengo Sakaguchi
Oct 17, 2008·The EMBO Journal·Tirzah Braz PettaPatricia Kannouche
May 26, 2006·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·Z M DongY L Wang
Mar 22, 2015·Mutation Research. Reviews in Mutation Research·Miral Dizdaroglu
Jul 9, 2005·The Journal of Biological Chemistry·Elena K BraithwaiteSamuel H Wilson
Feb 5, 2015·Physiological Reports·Virginijus ValiunasPeter R Brink

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