Knock-down of LRP/LR promotes apoptosis in early and late stage colorectal carcinoma cells via caspase activation

BMC Cancer
Leila VaniaStefan F T Weiss

Abstract

Cancer remains one of the leading causes of death around the world, where incidence and mortality rates are at a constant increase. Tumourigenic cells are characteristically seen to over-express the 37 kDa/67 kDa laminin receptor (LRP/LR) compared to their normal cell counterparts. This receptor has numerous roles in tumourigenesis including metastasis, angiogenic enhancement, telomerase activation, cell viability and apoptotic evasion. This study aimed to expose the role of LRP/LR on the cellular viability of early (SW-480) and late (DLD-1) stage colorectal cancer cells. siRNA were used to down-regulate the expression of LRP/LR in SW-480 and DLD-1 cells which was assessed using western blotting. Subsequently, cell survival was evaluated using the MTT cell survival assay and confocal microscopy. Thereafter, Annexin V-FITC/PI staining and caspase activity assays were used to investigate the mechanism underlying the cell death observed upon LRP/LR knockdown. The data was analysed using Student's t-test with a confidence interval of 95%, with p-values of less than 0.05 seen as significant. Here we reveal that siRNA-mediated knock-down of LRP led to notable decreases in cell viability through increased levels of apoptosis, apparent...Continue Reading

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Citations

Nov 13, 2019·Journal of Cellular Biochemistry·Mei TangHui Zhang
Nov 15, 2019·Expert Opinion on Therapeutic Patents·Leila VaniaStefan F T Weiss
Jun 23, 2020·Journal of Alzheimer's Disease : JAD·Katelyn CuttlerStefan F T Weiss

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Methods Mentioned

BETA
transfection
electrophoresis
transfections
PCA
confocal microscopy

Software Mentioned

Airyscan

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