Knock-down of RGS4 and beta tubulin in CHO cells expressing the human MT1 melatonin receptor prevents melatonin-induced receptor desensitization

Life Sciences
P A Witt-EnderbyMelissa A Melan

Abstract

Previously, it has been shown that chronic melatonin exposure in MT1-CHO cells results in receptor desensitization while at the same time producing drastic morphological changes. The addition of a depolymerizing agent during the melatonin pretreatment period prevents MT1 receptor desensitization and the changes in cellular morphology. The lack of morphological change in the presence of a depolymerizing agent is easily explained by the inability of the microtubules to polymerize, however, the prevention of receptor desensitization is a little more complex and may involve G-protein activation. The goal of this study was to determine whether melatonin-induced MT1 receptor desensitization is regulated by proteins known to regulate G-protein activation states, beta-tubulin and RGS4,using anti sense knockdown approaches. The expression of RGS4 mRNA in CHO cells was confirmed using RT PCR and successful knockdown of each was confirmed by western blot analysis or quantitative PCR. Pretreatment of MT1-CHO cells, transfected with the nonsense probes and exposed to melatonin, resulted in a desensitization of the receptor, an increase in forskolin-induced cAMP accumulation, an increase in 2-[125I]-iodomelatonin binding and no change in the...Continue Reading

References

Feb 1, 1994·Neurochemistry International·P J MorganR Helliwell
Nov 1, 1996·Biochemical Society Transactions·M J LohseC Krasel
Jan 21, 1997·Proceedings of the National Academy of Sciences of the United States of America·J R HeplerT Kozasa
Dec 5, 1997·Brain Research. Molecular Brain Research·J M Neu, L P Niles
May 9, 1998·Biochimica Et Biophysica Acta·N NishigakiM Negishi
May 1, 1999·The Journal of Biological Chemistry·S RoychowdhuryM M Rasenick
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·L De VriesM G Farquhar
Feb 24, 2001·The European Journal of Neuroscience·J GarzónP Sánchez-Blázquez
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 21, 2002·Biochimica Et Biophysica Acta·Janice L KongMichael T Greenwood
Feb 27, 2003·The Journal of Pharmacology and Experimental Therapeutics·M J GerdinM L Dubocovich
Mar 12, 2003·Life Sciences·Paula A Witt-EnderbyMelissa A Melan

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Citations

Apr 10, 2007·Journal of Molecular Neuroscience : MN·Avi RimlerNava Zisapel
Jul 23, 2011·Neuroendocrinology·Sandrine M Dupré
Feb 24, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yunjia ChenQin Wang
Nov 21, 2007·CNS Drugs·Seithikurippu R Pandi-PerumalDaniel P Cardinali
May 29, 2013·International Journal of Molecular Sciences·Di Yan LiQing Zhu
Jun 15, 2013·International Journal of Molecular Sciences·Domenico De BerardisMassimo di Giannantonio
May 29, 2021·Journal of Circadian Rhythms·Eric BarbatoThomas J Kelley

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