Knockdown of a mucin-like gene in Meloidogyne incognita (Nematoda) decreases attachment of endospores of Pasteuria penetrans to the infective juveniles and reduces nematode fecundity

Molecular Plant Pathology
Victor PhaniUma Rao

Abstract

Mucins are highly glycosylated polypeptides involved in many host-parasite interactions, but their function in plant-parasitic nematodes is still unknown. In this study, a mucin-like gene was cloned from Meloidogyne incognita (Mi-muc-1, 1125 bp) and characterized. The protein was found to be rich in serine and threonine with numerous O-glycosylation sites in the sequence. Quantitative real-time polymerase chain reaction (qRT-PCR) showed the highest expression in the adult female and in situ hybridization revealed the localization of Mi-muc-1 mRNA expression in the tail area in the region of the phasmid. Knockdown of Mi-muc-1 revealed a dual role: (1) immunologically, there was a significant decrease in attachment of Pasteuria penetrans endospores and a reduction in binding assays with human red blood cells (RBCs), suggesting that Mi-MUC-1 is a glycoprotein present on the surface coat of infective second-stage juveniles (J2s) and is involved in cellular adhesion to the cuticle of infective J2s; pretreatment of J2s with different carbohydrates indicated that the RBCs bind to J2 cuticle receptors different from those involved in the interaction of Pasteuria endospores with Mi-MUC-1; (2) the long-term effect of RNA interference (RN...Continue Reading

References

Jan 1, 1992·Critical Reviews in Biochemistry and Molecular Biology·G J Strous, J Dekker
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·D Gems, R M Maizels
Jul 20, 2000·Parasitology Today·A Loukas, R M Maizels
Nov 7, 2000·Parasitology Today·S J HicksA P Corfield
Apr 5, 2001·Trends in Parasitology·G TheodoropoulosS D Carrington
Jun 19, 2001·International Journal for Parasitology·R M MaizelsX Zang
Nov 15, 2001·Veterinary Parasitology·J A Appleton, F Romaris
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 7, 2002·Microbes and Infection·Jonathan J Ewbank
Jul 16, 2002·International Journal for Parasitology·Michael J KimberAaron G Maule
Aug 17, 2002·Molecular Plant-microbe Interactions : MPMI·P E UrwinHoward J Atkinson
Jun 18, 2004·Immunological Reviews·Hinrich SchulenburgJonathan J Ewbank
Jan 1, 1992·Parasitology Today·S M Politz, M Philipp
Jul 1, 1992·Parasitology Today·M L BlaxterR M Maizels
Mar 2, 2005·Trends in Microbiology·Costi D SifriFrederick M Ausubel
Aug 4, 2005·Journal of Bacteriology·Lauren CharlesCharles H Opperman
Apr 7, 2006·Applied and Environmental Microbiology·Silvia BulgheresiJörg A Ott
Nov 17, 2006·Nature·Jonathan D G Jones, Jeffery L Dangl
Dec 1, 2006·Advances in Parasitology·Patrick J Skelly, R Alan Wilson
Oct 24, 2007·Current Topics in Developmental Biology·Frédéric MarinDavorin Medakovic
Jul 29, 2008·Nature Biotechnology·Pierre AbadPatrick Wincker
Aug 25, 2009·Current Opinion in Structural Biology·Roland Schauer
Aug 26, 2009·Plant Signaling & Behavior·Marcello Iriti, Franco Faoro
Sep 16, 2009·Parasitology International·Yvonne HarcusRick M Maizels
Sep 23, 2009·Research in Microbiology·Laurence MoutonDieter Ebert
Feb 18, 2011·Gut Microbes·Muriel DerrienJan Dekker
Apr 5, 2011·Current Opinion in Plant Biology·Godelieve Gheysen, Melissa G Mitchum
May 17, 2011·Annual Review of Phytopathology·Keith G Davies, Rosane H C Curtis

❮ Previous
Next ❯

Datasets Mentioned

BETA
MG579969

Methods Mentioned

BETA
PCR
glycosylation

Software Mentioned

BLAST
BLASTX
BLASTN
ExPasy ProtParam
NetOGlyc
SAS
ExPasy ProtParam tool

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.