Knockdown of apolipoprotein B, an atherogenic apolipoprotein, in HepG2 cells by lentivirus-mediated siRNA

Biochemical and Biophysical Research Communications
Wei Liao, Guang Ning

Abstract

ApoB is an important determinant of atherosclerosis susceptibility and a potential pharmaceutical target for lowering atherogenic lipoproteins. In the present study, we used a lentiviral vector to express short hairpin RNAs for inhibition of apoB production in HepG2 cells. We first demonstrated that lentivirus could efficiently deliver transgene into HepG2 cells by using GFP lentivirus. We then made three lentiviral siApoB constructs, two of which were highly efficient for silencing apoB expression in HepG2 cells. We showed that siApoB lentivirus specifically knocked down apoB but had no effects on other proteins such as apoAI and albumin. Consequently, the secretion of apoB was reduced markedly. The silencing effect of siApoB lentivirus appeared to be permanent. Knocking down apoB did not alter the expression of cytoplasmic stress proteins (HSP70 and HSP90) and their ER homologues (GRP78 and GRP94). Furthermore, neither IKKalpha and JNK nor phosphorylated IKK and JNK were increased in long-term apoB-deficient hepatocytes as compared to the control cells. Consistent with these findings, apoB-deficient hepatocytes responded to insulin to a similar extent as the control cells as determined by measuring insulin-induced phosphoryla...Continue Reading

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Citations

Dec 14, 2007·Journal of Virology·Pablo GastaminzaFrancis V Chisari
Mar 7, 2014·Cytotechnology·Masoud NasriMehdi Allahbakhshian Farsani
Jun 19, 2008·Biochemical and Biophysical Research Communications·Wei LiaoLawrence Chan
May 13, 2008·Chemico-biological Interactions·Stephanie M ZamuleCurtis J Omiecinski
Sep 28, 2016·Expert Opinion on Drug Metabolism & Toxicology·M José Gómez-LechónM Teresa Donato

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