Knockdown of arylalkylamine N-acetyltransferase-like 2 in Drosophila melanogaster

Archives of Insect Biochemistry and Physiology
Ryan L AndersonDavid J Merkler

Abstract

Drosophila melanogaster produces fatty acid amides, and thus, provides a model to unravel the pathways for their biosynthesis. We previously demonstrated that arylalkylamine N-acetyltransferase-like 2 (AANATL2) from D. melanogaster will catalyze the formation of long-chain N-acylserotonins and N-acyldopamines in vitro. Generating silencing RNA via the UAS/GAL4 bipartite approach for targeted gene expression effectively decreased the endogenous levels of the AANATL2 transcripts in D. melanogaster, as shown by reverse transcription quantitative polymerase chain reaction. Consistent with these data, western blot analysis of the offspring of the AANATL2 knockdown flies using an anti-AANATL2 antibody revealed a significant reduction in the expression of the AANATL2 protein. Reduced expression of AANATL2 decreased the cellular levels of N-palmitoyldopamine (PALDA), providing strong evidence that AANATL2 is responsible for the biosynthesis of PALDA in vivo. This is the first time that the expression of an AANAT has been reduced in D. melanogaster to link one of these enzymes to the in vivo production of an N-acylarylalkylamide.

References

Oct 4, 1995·Biochemical and Biophysical Research Communications·T SugiuraK Waku
Jul 12, 2001·The Journal of Comparative Neurology·J McPartlandM Glass
Sep 27, 2002·Genesis : the Journal of Genetics and Development·Joseph B Duffy
Feb 6, 2003·The Journal of Biological Chemistry·Constance J ChuJ Michael Walker
Jun 19, 2004·Science·Lawrence Lum, Philip A Beachy
Dec 8, 2004·Archives of Biochemistry and Biophysics·Matthew W VettingJohn S Blanchard
Aug 16, 2005·Prostaglandins & Other Lipid Mediators·J Michael WalkerSusan M Huang
Nov 4, 2005·Journal of Chromatography. a·Tamanna Sultana, Mitchell E Johnson
Jul 5, 2008·Drug Discovery Today·Emma K Farrell, David J Merkler
Apr 22, 2009·Prostaglandins & Other Lipid Mediators·Heather B BradshawDouglas McHugh
Mar 5, 2010·BMC Research Notes·Katherine HolmesMichael J Cross
Mar 22, 2014·FEBS Letters·Kristen A JeffriesDavid J Merkler
Mar 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Helena KhaliullinaSuzanne Eaton
Sep 2, 2015·European Journal of Pharmacology·Renger Witkamp
Mar 12, 2016·Progress in Lipid Research·Fabio Arturo IannottiStefania Petrosino
Jul 2, 2016·International Journal of Molecular Sciences·Abu Iftiaf Md Salah Ud-DinAnna Roujeinikova
Aug 11, 2018·Frontiers in Molecular Biosciences·Brian G O'FlynnDavid J Merkler
Aug 15, 2018·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Ryan L AndersonDavid J Merkler
Feb 19, 2019·Trends in Research·David J Merkler, James W Leahy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.