Knockdown of CLIC4 enhances ATP-induced HN4 cell apoptosis through mitochondrial and endoplasmic reticulum pathways

Cell & Bioscience
Haowei XueBing Shen

Abstract

Human head and neck squamous carcinoma is the 6th most prevalent carcinoma worldwide. Although many novel therapies have been developed, the clinical treatment for patients remains non-ideal. Chloride intracellular channel 4 (CLIC4), one of the seven members of the CLIC family, is a newly found Cl(-) channel that participates in various biological processes, including cellular apoptosis and differentiation. Accumulating evidence has revealed the significant role of CLIC4 in regulating the apoptosis of different cancer cells. Here, we investigated the functional role of CLIC4 in the apoptosis of HN4 cells, a human head and neck squamous carcinoma cell line. In the present study, we used immunohistochemical staining to demonstrate that the expression level of CLIC4 is elevated in the tissue of human oral squamous carcinoma compared with healthy human gingival tissue. Specific CLIC4 small interfering RNA was used to knockdown the expression of CLIC4. The results showed that knockdown of CLIC4 with or without 100 μM adenosine triphosphate (ATP) treatment significantly increased the expression of Bax, active caspase 3, active caspase 4 and CHOP but suppressed Bcl-2 expression in HN4 cells. Moreover, the results from the TdT-mediated...Continue Reading

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Citations

Jul 16, 2020·Brazilian Dental Journal·Francisco Jadson LimaÉricka Janine Dantas da Silveira
Oct 4, 2017·World Journal of Gastroenterology : WJG·Fu-Rong WangYu-Min Li
Mar 3, 2020·Frontiers in Physiology·Shubha Gururaja RaoHarpreet Singh
Oct 25, 2020·Cancers·Michaela GriffinStuart Smith
Mar 12, 2021·Nutrition and Cancer·Reo YokoyamaMasaru Terasaki
Feb 15, 2020·British Journal of Pharmacology·Umberto De MarchiJaime Santo-Domingo

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Methods Mentioned

BETA
transfection
nuclear translocation
fluorescence microscopy

Software Mentioned

SigmaPlot

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