Knockdown of CREB1 promotes apoptosis and decreases estradiol synthesis in mouse granulosa cells

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Pengju ZhangXinghong Wu

Abstract

Cyclic AMP response element-binding protein 1 (CREB1), a member of the CREB family, is known to be involved in follicular growth, ovulation, and ovarian disease. However, the physiological function of CREB1 in mouse granulosa cells (mGCs) remains lagerly unknown. The aim of this study was to determine the role of CREB1 in mGCs by knocking down CREB1 expression. CREB1 knock-down in mGCs at the mRNA and protein levels, was confirmed by quantitative real-time polymerase chain reaction and western blot. Results of enzyme linked immunosorbent assay revealed that CREB1 knockdown significantly decreased the concentrations of estradiol (E2) and progesterone (P4) in mGCs. Furthermore, the CREB1 knockdown in mGCs promoted cell proliferation and apoptosis, and arrested the cell cycle in S-phase. To elucidate the regulatory mechanism underlying the effects of CREB1 knockdown on steroid synthesis, cell cycle, and apoptosis, we measured the protein expression levels of several related genes in mGCs knocked down CREB1. When CREB1 was knocked down, the expression of Cyp1b1 and Cyp19a1, which encode steroidogenic enzymes, was down-regulated; the expression of the cell cycle factors CyclinA1, CyclinB1, and CyclinD2 were significantly decreased. ...Continue Reading

References

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Citations

Dec 18, 2018·Journal of Cellular Biochemistry·Pengju ZhangXinghong Wu
Nov 27, 2019·Biomolecules·Laith N Al-EitanFiras A Al-Qarqaz
Sep 5, 2019·Asian-Australasian Journal of Animal Sciences·Xiaofeng ZhouJiaqi Li
Jun 5, 2020·Histochemistry and Cell Biology·Jia LiMeijia Zhang
Feb 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yongjie XiongShaojun He
Dec 29, 2020·Ecotoxicology and Environmental Safety·Zhang YimingLi Shu
Aug 30, 2019·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Xiao-Shuang WangDe-Shou Wang
Nov 17, 2021·Journal of Zhejiang University. Science. B·Zhinan ZhouLei Hong

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