Knockdown of FBXO39 inhibits proliferation and promotes apoptosis of human osteosarcoma U-2OS cells

Oncology Letters
Jianrong ZhengShiquan Zhang

Abstract

F-box proteins are essential components of the Skp-cullin-F-box complex (a type of E3 ubiquitin ligase), and participate in cell cycle and immune responses through the ubiquitin proteasome system. F-box protein 39 (FBXO39) belongs to the F-box family, which has been reported to be associated with cancer oncogenesis and progression. The present study aimed to investigate the role of FBXO39 in osteosarcoma (OS) cell proliferation and apoptosis in vitro. It was demonstrated that U-2OS cells exhibited high expression of FBXO39 compared with HOS and SaOS-2 osteosarcoma cells. Thus, knockdown of FBXO39 was performed using lentivirus-mediated short hairpin RNA (shRNA) transfection to validate the effect of FBXO39 in U-2OS cells. Western blotting and RT-qPCR analysis were used to confirm the efficiency of infection by analyzing the expression level of FBXO39. Using Celigo-based cell counting and MTT assays, it was demonstrated that FBXO39 knockdown significantly reduced the rate of cell proliferation compared with control. Caspase 3/7 activity assays and fluorescence-activated cell sorting confirmed the induction of apoptosis in U-2OS cells following FBXO39 knockdown. In conclusion, it was demonstrated that FBXO39 knockdown may signifi...Continue Reading

References

May 1, 1997·Cancer Genetics and Cytogenetics·J A BridgeJ R Neff
Feb 24, 2001·Genome Biology·E T Kipreos, M Pagano
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 3, 2003·Nature Reviews. Cancer·Lee J Helman, Paul Meltzer
Jul 22, 2004·The Oncologist·Neyssa MarinaRichard Gorlick
Nov 3, 2004·Genes & Development·Jianping JinJ Wade Harper
Mar 18, 2008·Cellular and Molecular Life Sciences : CMLS·M S HoH Pi
May 8, 2009·Current Opinion in Pediatrics·Travis HeareShelley Dell'Orfano
Mar 10, 2010·Cancer Treatment and Research·Giulia Ottaviani, Norman Jaffe
Jul 9, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jan SmidaMichaela Nathrath
Feb 23, 2011·Biochemical and Biophysical Research Communications·Myung-Ha SongSang-Yull Lee
Apr 27, 2011·Journal of Translational Medicine·Christoph S NabzdykFrank W Logerfo
Apr 5, 2012·Molecular and Cellular Biology·Jonathan E GrimBruce E Clurman
Jun 5, 2012·Cancer Letters·Upal Basu-RoyAlka Mansukhani
Nov 13, 2013·Cancers·Kosei AndoDominique Heymann
Jan 1, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Mahnaz Seifi-AlanMitra Najafi-Ashtiani
Mar 8, 2014·Molecular Medicine Reports·Feng LinYang Yao
Jul 30, 2014·Cancer Treatment and Research·Drew D Moore, Hue H Luu
Sep 28, 2015·Seminars in Cancer Biology·Shahab UddinRamzi M Mohammad
Apr 5, 2016·Cancer Biotherapy & Radiopharmaceuticals·Weilin SangCong Wang

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Citations

Sep 19, 2019·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Zhi LiuJian Shui Li

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