PMID: 32519669Jun 11, 2020Paper

Knockdown of Fez family zinc finger protein 1 antisense ribonucleic acid 1 (FEZF1-AS1) inhibits invasion and migration of esophageal squamous cell carcinoma cells by blocking JAK2/STAT3 pathway

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Yitong CaoLi Chen

Abstract

Objective To investigate the relationship between long chain non-coding RNA Fez family zinc finger protein 1 antisense ribonucleic acid 1 (FEZF1-AS1) and invasion, migration and Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signal pathway in esophageal squamous cell carcinoma (ESCC) cells. Methods The expression' of FEZF1-AS1 in ESCC cancer tissue and ESCC cell line was detected by real-time quantitative PCR. After knocking down the FEZF1-AS1 of KYSE150 and KYSE510 cells by the constructed lentiviral expression vector, the changes of cell cycle were detected by flow cytometry, the ability of cell proliferation by colony formation assay, the ability of cell invasion by TranswellTM invasion assay, the ability of cell migration by TranswellTM migration and scratch assay, and the protein levels of JAK2 and phosphorylated STAT3 (p-STAT3) by Western blotting. Results FEZF1-AS1 was highly expressed in ESCC cancer tissues, and FEZF1-AS1 expression in KYSE150 and KYSE510 cells were higher than that in normal esophageal squamous epithelial cells. After the knockdown of FEZF1-AS1 expression, the cell cycle and proliferation of ESCC cancer cells had no obvious change, but cell invasion and migration were si...Continue Reading

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