Knockdown of Five Genes Encoding Uncharacterized Proteins Inhibits Entamoeba histolytica Phagocytosis of Dead Host Cells

Infection and Immunity
Adam SaterialeChristopher D Huston

Abstract

Entamoeba histolytica is the protozoan parasite that causes invasive amebiasis, which is endemic to many developing countries and characterized by dysentery and liver abscesses. The virulence of E. histolytica correlates with the degree of host cell engulfment, or phagocytosis, and E. histolytica phagocytosis alters amebic gene expression in a feed-forward manner that results in an increased phagocytic ability. Here, we used a streamlined RNA interference screen to silence the expression of 15 genes whose expression was upregulated in phagocytic E. histolytica trophozoites to determine whether these genes actually function in the phagocytic process. When five of these genes were silenced, amebic strains with significant decreases in the ability to phagocytose apoptotic host cells were produced. Phagocytosis of live host cells, however, was largely unchanged, and the defects were surprisingly specific for phagocytosis. Two of the five encoded proteins, which we named E. histolytica ILWEQ (EhILWEQ) and E. histolytica BAR (EhBAR), were chosen for localization via SNAP tag labeling and localized to the site of partially formed phagosomes. Therefore, both EhILWEQ and EhBAR appear to contribute to E. histolytica virulence through the...Continue Reading

References

Nov 1, 1978·The Journal of Experimental Medicine·D TrisslE Pérez de Suárez
Sep 1, 1985·The Journal of Infectious Diseases·J I RavdinS A Long-Krug
Jul 1, 1986·The Journal of Infectious Diseases·M A Rodríguez, E Orozco
Aug 1, 1980·The Journal of Experimental Medicine·J I RavdinR L Guerrant
Nov 1, 1983·The Journal of Experimental Medicine·E OrozcoT Sánchez
Mar 1, 1994·Journal of Clinical Pathology·A González-RuizD C Warhurst
May 27, 1997·Proceedings of the National Academy of Sciences of the United States of America·R O McCann, S W Craig
Nov 5, 1997·Lancet·S Hohenschild
Jul 14, 1999·The American Journal of Tropical Medicine and Hygiene·R HaqueW A Petri
Feb 24, 2001·Cellular Microbiology·C D HustonW A Petri
Jan 24, 2003·Infection and Immunity·Christopher D HustonWilliam A Petri
Apr 18, 2003·The New England Journal of Medicine·Rashidul HaqueWilliam A Petri
Oct 9, 2003·Journal of Clinical Microbiology·Joerg BlessmannEgbert Tannich
Dec 3, 2003·Science·Brian J PeterHarvey T McMahon
Dec 6, 2003·Journal of Cell Science·Valerie Legendre-GuilleminPeter S McPherson
May 1, 1997·Parasitology Today·M Leippe
Nov 3, 2004·Nature Reviews. Molecular Cell Biology·Julie L Kadrmas, Mary C Beckerle
May 31, 2006·PLoS Pathogens·Rivka BrachaDavid Mirelman
Jun 7, 2006·Proceedings of the National Academy of Sciences of the United States of America·Martin E LidellGunnar C Hansson
Nov 16, 2007·Proteins·K AnamikaN Srinivasan
Dec 19, 2007·Infection and Immunity·Jose E Teixeira, Christopher D Huston
Jan 25, 2008·Nature Reviews. Molecular Cell Biology·Mark A Lemmon
Aug 16, 2008·The Journal of Infectious Diseases·Jose Eduardo TeixeiraChristopher Dwight Huston
Oct 28, 2008·Current Opinion in Structural Biology·Björn SjöblomKristina Djinović-Carugo
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Jan 20, 2010·International Journal for Parasitology·Sarah N BussWilliam A Petri

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Citations

Jun 9, 2020·Proteins·Pragyan Parimita Rath, Samudrala Gourinath
Jun 7, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Zijian LingXunjia Cheng
Sep 7, 2021·FEBS Open Bio·América Itzallana Salgado-MartínezLaurence A Marchat

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