Knockdown of flotillin-2 impairs the proliferation of breast cancer cells through modulation of Akt/FOXO signaling

Oncology Reports
Gui'e XieCan Liao

Abstract

Lipid rafts, specialized domains in cell membranes, function as physical platforms for various molecules to coordinate a variety of signal transduction processes. Flotillin-2 (FLOT2), a marker of lipid rafts, is involved in the progression of cancer, yet the precise mechanism remains unclear. In the present study, we examined the effect of FLOT2 on cell proliferation and found that silencing endogenous FLOT2 with shRNAs inhibited proliferation of breast cancer cells. Furthermore, the antiproliferative effect of silencing FLOT2 on breast cancer cells was associated with upregulation of cyclin-dependent kinase (CDK) inhibitors p21Cip1 and p27Kip1. Moreover, we further demonstrated that the silencing of FLOT2 enhanced the transcriptional activity of FOXO factors by decreasing its phosphorylation through inhibiting the PI3K/Akt signaling pathway. Taken together, our results provide the first demonstration of a novel mechanism by which FLOT2 induces proliferation of breast cancer cells, and our findings suggest that FLOT2 plays an important role in oncogenesis of breast cancer and thereby may be a potential target for human breast cancer treatment.

References

Nov 16, 2001·Annals of the New York Academy of Sciences·W DoerflerJ Hertz
Mar 9, 2002·Molecular and Cellular Biology·William C HahnRobert A Weinberg
Sep 2, 2004·Nature Reviews. Molecular Cell Biology·Timothy Cardozo, Michele Pagano
Jan 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Haojie HuangDonald J Tindall
Aug 11, 2005·Cellular and Molecular Life Sciences : CMLS·M F LanghorstC A O Stuermer
May 8, 2007·European Journal of Cell Biology·Tanja Babuke, Ritva Tikkanen
Apr 9, 2008·Oncogene·B M Th Burgering
Jan 29, 2010·PloS One·Sascha PustKirsten Sandvig
Jan 22, 2011·Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer·Christine M Friedenreich
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Mar 31, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Chuyong LinLibing Song
Aug 16, 2013·Journal of Translational Medicine·Xi WangHuan-Xin Lin
Jul 24, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yang YanJun-Hua Zheng
Aug 7, 2014·International Journal of Oncology·See-Hyoung ParkMickey C-T Hu

❮ Previous
Next ❯

Citations

Mar 6, 2018·Journal of Zhejiang University. Science. B·Xu-Xu LiuCai-Ping Ren
May 16, 2017·Artificial Cells, Nanomedicine, and Biotechnology·Guangxia WeiJie Yan
Apr 1, 2020·International Journal of Molecular Sciences·Katarzyna KwiatkowskaAnna Ciesielska
Aug 5, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·An-Ting LiouChu-Wen Yang
Jan 22, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Shaosong HuangWei Lin
Feb 26, 2021·Biochemical and Biophysical Research Communications·Tao SongWei Huang

❮ Previous
Next ❯

Related Concepts

Related Feeds

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.