Knockdown of glutamate cysteine ligase catalytic subunit by siRNA causes the gold nanoparticles-induced cytotoxicity in lung cancer cells

PloS One
Min LiuXiumei Zhang

Abstract

Gold nanoparticles (GNPs) have shown promising medical applications in cancer treatment involved in the regulation of intracellular redox balance. Previously, we have reported that GNPs can trigger apoptosis and necrosis in human lung cancer cells (A549) when L-buthionine-sulfoximine (BSO) was used to decrease the expression of intracellular glutathione (GSH). Herein, we investigated the cytotoxicity of GNPs toward lung cancer cells under the glutamate cysteine ligase catalytic subunit (GCLC) was silenced by siRNA. Our results showed that GNPs cause apoptosis and necrosis in cells transfected with GCLC siRNA by elevating intracellular reactive oxygen species (ROS). These findings demonstrated that the regulation of glutathione synthesis by GCLC siRNA in A549 cells can initiate the gold nanoparticles-induced cytotoxicity.

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Citations

Mar 10, 2018·International Journal of Molecular Sciences·Hainan SunShumei Zhai
Jul 10, 2019·Oxidative Medicine and Cellular Longevity·Huanhuan LvPeng Shang
Dec 4, 2020·Emerging Topics in Life Sciences·Ainoa GuinartTeresa D Tetley
Sep 26, 2020·Annual Review of Pharmacology and Toxicology·Tsuyoshi Yokoi, Shingo Oda

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Methods Mentioned

BETA
Transfection
PCR
flow cytometry

Software Mentioned

GraphPad
Prism
Cell Quest

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