Knockdown of HBV surface antigen gene expression by a lentiviral microRNA-based system inhibits HBV replication and HCC growth

Journal of Viral Hepatitis
L XiangjiW Mengchao

Abstract

Current options for the treatment of hepatitis B virus (HBV) infections, a common liver cancer risk factor, are limited. While RNA interference (RNAi) technologies have been shown to inhibit HBV replication, the consequent effects on hepatocellular carcinoma (HCC) cell growth are not fully understood. The aim of this study was to evaluate the effect of RNAi-mediated decrease in the HBV surface antigen (HBsAg) gene on HBV replication and HCC growth. A lentiviral microRNA-based system expressing siRNAs targeting the HBsAg gene (LVshHBS) was developed and transfected into HepG2.2.15 cells (HBV stably expressing line). We found that LVshHBS significantly inhibited the HBsAg mRNA and protein levels in the HepG2.2.15 cells, while HBsAg secretion into the culture supernatant decreased by 70%. BALB/c (nu/nu) mice were injected with HepG2.2.15 cells transduced with LVshHBS or control vectors to investigate the effect of inhibiting the HBsAg on the development of tumour growth in a human HCC nude mice model. Compared with the control, the tumour growth in nude mice was significantly decreased after injection with LVshHBS. Microarray analysis of tumour-related genes in LVshHBS-transduced HepG2.2.15 cells showed that the expressions of gen...Continue Reading

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Citations

Nov 2, 2012·Cancer Metastasis Reviews·Mausumi BharadwajShowket Hussain
Feb 15, 2011·Nucleic Acids Research·Nicoletta PotenzaAniello Russo
Aug 16, 2013·World Journal of Gastroenterology : WJG·Ying-Feng WeiHong-Yan Diao
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Mar 3, 2015·World Journal of Hepatology·Maren GebbingAnja Ehrhardt
Jan 29, 2016·Biomarker Insights·Jennifer LoutenGabrielle Holenstein
Oct 14, 2018·Laboratory Investigation; a Journal of Technical Methods and Pathology·Yuhui FanMei Liu

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